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CUT&RUN detects distinct DNA footprints of RNA polymerase II near the transcription start sites

CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in a multi-well cell cultu...

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Detalles Bibliográficos
Autores principales: Miura, Michi, Chen, Honglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691310/
https://www.ncbi.nlm.nih.gov/pubmed/33070289
http://dx.doi.org/10.1007/s10577-020-09643-0