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A Novel FRET Approach Quantifies the Interaction Strength of Peroxisomal Targeting Signals and Their Receptor in Living Cells

Measuring Förster–resonance–energy–transfer (FRET) efficiency allows the investigation of protein–protein interactions (PPI), but extracting quantitative measures of affinity necessitates highly advanced technical equipment or isolated proteins. We demonstrate the validity of a recently suggested no...

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Detalles Bibliográficos
Autores principales: Hochreiter, Bernhard, Chong, Cheng-Shoong, Hartig, Andreas, Maurer-Stroh, Sebastian, Berger, Johannes, Schmid, Johannes A., Kunze, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693011/
https://www.ncbi.nlm.nih.gov/pubmed/33143123
http://dx.doi.org/10.3390/cells9112381