Cargando…

A Novel FRET Approach Quantifies the Interaction Strength of Peroxisomal Targeting Signals and Their Receptor in Living Cells

Measuring Förster–resonance–energy–transfer (FRET) efficiency allows the investigation of protein–protein interactions (PPI), but extracting quantitative measures of affinity necessitates highly advanced technical equipment or isolated proteins. We demonstrate the validity of a recently suggested no...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hochreiter, Bernhard, Chong, Cheng-Shoong, Hartig, Andreas, Maurer-Stroh, Sebastian, Berger, Johannes, Schmid, Johannes A., Kunze, Markus
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693011/ https://www.ncbi.nlm.nih.gov/pubmed/33143123 http://dx.doi.org/10.3390/cells9112381

Ejemplares similares

Rare Human Missense Variants can affect the Function of Disease-Relevant Proteins by Loss and Gain of Peroxisomal Targeting Motifs
por: Chong, Cheng-Shoong, et al.
Publicado: (2019)

Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells
por: Hochreiter, Bernhard, et al.
Publicado: (2019)

Hidden localization motifs: naturally occurring peroxisomal targeting signals in non-peroxisomal proteins
por: Neuberger, Georg, et al.
Publicado: (2004)

Studying the interaction between PEX5 and its full-length cargo proteins in living cells by a novel Försteŕs resonance energy transfer-based competition assay
por: Hochreiter, Bernhard, et al.
Publicado: (2022)

Fluorescent Proteins as Genetically Encoded FRET Biosensors in Life Sciences
por: Hochreiter, Bernhard, et al.
Publicado: (2015)

Permeability of the peroxisomal membrane: lessons from the glyoxylate cycle
por: Kunze, Markus, et al.
Publicado: (2013)

Addendum: Hochreiter, B.; Pardo-Garcia, A.; Schmid, J.A. Fluorescent Proteins as Genetically Encoded FRET Biosensors in Life Sciences. Sensors 2015, 15, 26281–26314
por: Hochreiter, Bernhard, et al.
Publicado: (2015)

Peroxisomes in brain development and function☆
por: Berger, Johannes, et al.
Publicado: (2015)

Structure-based virtual screening of CYP1A1 inhibitors: towards rapid tier-one assessment of potential developmental toxicants
por: Goh, Janice Jia Ni, et al.
Publicado: (2021)

Extracellular Vesicles Linking Inflammation, Cancer and Thrombotic Risks
por: Beck, Sarah, et al.
Publicado: (2022)

A Subtle Interplay Between Three Pex11 Proteins Shapes De Novo Formation and Fission of Peroxisomes
por: Huber, Anja, et al.
Publicado: (2012)

Fluorescence colocalization microscopy analysis can be improved by combining object‐recognition with pixel‐intensity‐correlation
por: Moser, Bernhard, et al.
Publicado: (2016)

Integrity of lipid nanocarriers in bloodstream and tumor quantified by near-infrared ratiometric FRET imaging in living mice
por: Bouchaala, Redouane, et al.
Publicado: (2016)

QuanTI-FRET: a framework for quantitative FRET measurements in living cells
por: Coullomb, Alexis, et al.
Publicado: (2020)

Insights Into the Peroxisomal Protein Inventory of Zebrafish
por: Kamoshita, Maki, et al.
Publicado: (2022)

The similarity between N-terminal targeting signals for protein import into different organelles and its evolutionary relevance
por: Kunze, Markus, et al.
Publicado: (2015)

Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells
por: Liu, Boqun, et al.
Publicado: (2018)

Homotransfer FRET Reporters for Live Cell Imaging
por: Snell, Nicole E., et al.
Publicado: (2018)

Fretting and Corrosion in Modular Shoulder Arthroplasty: A Retrieval Analysis
por: Eckert, Johannes A., et al.
Publicado: (2016)

Quantifying autophagy using novel LC3B and p62 TR-FRET assays
por: Bresciani, Alberto, et al.
Publicado: (2018)

Peroxisomal alterations in Alzheimer’s disease
por: Kou, Jianqiu, et al.
Publicado: (2011)

Novel approach for accurate tissue-based protein colocalization and proximity microscopy
por: Lutz, Mirjam I., et al.
Publicado: (2017)

Correction to “Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells”
por: Liu, Boqun, et al.
Publicado: (2020)

A Method to Quantify FRET Stoichiometry with Phasor Plot Analysis and Acceptor Lifetime Ingrowth
por: Chen, WeiYue, et al.
Publicado: (2015)

Development of Diubiquitin‐Based FRET Probes To Quantify Ubiquitin Linkage Specificity of Deubiquitinating Enzymes
por: Geurink, Paul P., et al.
Publicado: (2016)

Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET
por: Madeira, Catarina, et al.
Publicado: (2008)

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
por: Axmann, Markus, et al.
Publicado: (2015)

Peroxisomal very long-chain fatty acid transport is targeted by herpesviruses and the antiviral host response
por: Weinhofer, Isabelle, et al.
Publicado: (2022)

N-Way FRET Microscopy of Multiple Protein-Protein Interactions in Live Cells
por: Hoppe, Adam D., et al.
Publicado: (2013)

BOBA FRET: Bootstrap-Based Analysis of Single-Molecule FRET Data
por: König, Sebastian L. B., et al.
Publicado: (2013)

Flow cytometry-based FRET identifies binding intensities in PPARγ1 protein-protein interactions in living cells
por: Trümper, Verena, et al.
Publicado: (2019)

Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations
por: Costello, Joseph L., et al.
Publicado: (2017)

DeepFRET, a software for rapid and automated single-molecule FRET data classification using deep learning
por: Thomsen, Johannes, et al.
Publicado: (2020)

Sequence-function correlations and dynamics of ERG isoforms. ERG8 is the black sheep of the family
por: Hoesel, Bastian, et al.
Publicado: (2015)

Antagonistic Functions of Androgen Receptor and NF-κB in Prostate Cancer—Experimental and Computational Analyses
por: Basílio, José, et al.
Publicado: (2022)

Dual targeting of peroxisomal proteins
por: Ast, Julia, et al.
Publicado: (2013)

Increase in Strength and Fretting Resistance of Alloy 718 Using the Surface Modification Process
por: Amanov, Auezhan, et al.
Publicado: (2018)

Detecting stoichiometry of macromolecular complexes in live cells using FRET
por: Ben-Johny, Manu, et al.
Publicado: (2016)

A Protocol for FRET-Based Live-Cell Imaging in Microglia
por: Socodato, Renato, et al.
Publicado: (2020)

Current advances in the function and biogenesis of peroxisomes and their roles in health and disease
por: Dahan, Noa, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_c4er6nk406svih0a69i01n8qa2