Cargando…

Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages

BACKGROUND: Liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs; liver resident macrophages) form the body’s most effective scavenger cell system for the removal of harmful blood-borne substances, ranging from modified self-proteins to pathogens and xenobiotics. Controversies in the li...

Descripción completa

Detalles Bibliográficos
Autores principales: Bhandari, Sabin, Li, Ruomei, Simón-Santamaría, Jaione, McCourt, Peter, Johansen, Steinar Daae, Smedsrød, Bård, Martinez-Zubiaurre, Inigo, Sørensen, Karen Kristine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694354/
https://www.ncbi.nlm.nih.gov/pubmed/33246411
http://dx.doi.org/10.1186/s12860-020-00331-9
_version_ 1783614957967900672
author Bhandari, Sabin
Li, Ruomei
Simón-Santamaría, Jaione
McCourt, Peter
Johansen, Steinar Daae
Smedsrød, Bård
Martinez-Zubiaurre, Inigo
Sørensen, Karen Kristine
author_facet Bhandari, Sabin
Li, Ruomei
Simón-Santamaría, Jaione
McCourt, Peter
Johansen, Steinar Daae
Smedsrød, Bård
Martinez-Zubiaurre, Inigo
Sørensen, Karen Kristine
author_sort Bhandari, Sabin
collection PubMed
description BACKGROUND: Liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs; liver resident macrophages) form the body’s most effective scavenger cell system for the removal of harmful blood-borne substances, ranging from modified self-proteins to pathogens and xenobiotics. Controversies in the literature regarding the LSEC phenotype pose a challenge when determining distinct functionalities of KCs and LSECs. This may be due to overlapping functions of the two cells, insufficient purification and/or identification of the cells, rapid dedifferentiation of LSECs in vitro, or species differences. We therefore characterized and quantitatively compared expressed gene products of freshly isolated, highly pure LSECs (fenestrated SE-1/FcγRIIb2(+)) and KCs (CD11b/c(+)) from Sprague Dawley, Crl:CD (SD), male rats using high throughput mRNA-sequencing and label-free proteomics. RESULTS: We observed a robust correlation between the proteomes and transcriptomes of the two cell types. Integrative analysis of the global molecular profile demonstrated the immunological aspects of LSECs. The constitutive expression of several immune genes and corresponding proteins of LSECs bore some resemblance with the expression in macrophages. LSECs and KCs both expressed high levels of scavenger receptors (SR) and C-type lectins. Equivalent expression of SR-A1 (Msr1), mannose receptor (Mrc1), SR-B1 (Scarb1), and SR-B3 (Scarb2) suggested functional similarity between the two cell types, while functional distinction between the cells was evidenced by LSEC-specific expression of the SRs stabilin-1 (Stab1) and stabilin-2 (Stab2), and the C-type lectins LSECtin (Clec4g) and DC-SIGNR (Clec4m). Many immune regulatory factors were differentially expressed in LSECs and KCs, with one cell predominantly expressing a specific cytokine/chemokine and the other cell the cognate receptor, illustrating the complex cytokine milieu of the sinusoids. Both cells expressed genes and proteins involved in antigen processing and presentation, and lymphocyte co-stimulation. CONCLUSIONS: Our findings support complementary and partly overlapping scavenging and immune functions of LSECs and KCs. This highlights the importance of including LSECs in studies of liver immunity, and liver clearance and toxicity of large molecule drugs and nano-formulations. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12860-020-00331-9.
format Online
Article
Text
id pubmed-7694354
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-76943542020-11-30 Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages Bhandari, Sabin Li, Ruomei Simón-Santamaría, Jaione McCourt, Peter Johansen, Steinar Daae Smedsrød, Bård Martinez-Zubiaurre, Inigo Sørensen, Karen Kristine BMC Mol Cell Biol Research Article BACKGROUND: Liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs; liver resident macrophages) form the body’s most effective scavenger cell system for the removal of harmful blood-borne substances, ranging from modified self-proteins to pathogens and xenobiotics. Controversies in the literature regarding the LSEC phenotype pose a challenge when determining distinct functionalities of KCs and LSECs. This may be due to overlapping functions of the two cells, insufficient purification and/or identification of the cells, rapid dedifferentiation of LSECs in vitro, or species differences. We therefore characterized and quantitatively compared expressed gene products of freshly isolated, highly pure LSECs (fenestrated SE-1/FcγRIIb2(+)) and KCs (CD11b/c(+)) from Sprague Dawley, Crl:CD (SD), male rats using high throughput mRNA-sequencing and label-free proteomics. RESULTS: We observed a robust correlation between the proteomes and transcriptomes of the two cell types. Integrative analysis of the global molecular profile demonstrated the immunological aspects of LSECs. The constitutive expression of several immune genes and corresponding proteins of LSECs bore some resemblance with the expression in macrophages. LSECs and KCs both expressed high levels of scavenger receptors (SR) and C-type lectins. Equivalent expression of SR-A1 (Msr1), mannose receptor (Mrc1), SR-B1 (Scarb1), and SR-B3 (Scarb2) suggested functional similarity between the two cell types, while functional distinction between the cells was evidenced by LSEC-specific expression of the SRs stabilin-1 (Stab1) and stabilin-2 (Stab2), and the C-type lectins LSECtin (Clec4g) and DC-SIGNR (Clec4m). Many immune regulatory factors were differentially expressed in LSECs and KCs, with one cell predominantly expressing a specific cytokine/chemokine and the other cell the cognate receptor, illustrating the complex cytokine milieu of the sinusoids. Both cells expressed genes and proteins involved in antigen processing and presentation, and lymphocyte co-stimulation. CONCLUSIONS: Our findings support complementary and partly overlapping scavenging and immune functions of LSECs and KCs. This highlights the importance of including LSECs in studies of liver immunity, and liver clearance and toxicity of large molecule drugs and nano-formulations. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12860-020-00331-9. BioMed Central 2020-11-27 /pmc/articles/PMC7694354/ /pubmed/33246411 http://dx.doi.org/10.1186/s12860-020-00331-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Bhandari, Sabin
Li, Ruomei
Simón-Santamaría, Jaione
McCourt, Peter
Johansen, Steinar Daae
Smedsrød, Bård
Martinez-Zubiaurre, Inigo
Sørensen, Karen Kristine
Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages
title Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages
title_full Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages
title_fullStr Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages
title_full_unstemmed Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages
title_short Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages
title_sort transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694354/
https://www.ncbi.nlm.nih.gov/pubmed/33246411
http://dx.doi.org/10.1186/s12860-020-00331-9
work_keys_str_mv AT bhandarisabin transcriptomeandproteomeprofilingrevealcomplementaryscavengerandimmunefeaturesofratliversinusoidalendothelialcellsandlivermacrophages
AT liruomei transcriptomeandproteomeprofilingrevealcomplementaryscavengerandimmunefeaturesofratliversinusoidalendothelialcellsandlivermacrophages
AT simonsantamariajaione transcriptomeandproteomeprofilingrevealcomplementaryscavengerandimmunefeaturesofratliversinusoidalendothelialcellsandlivermacrophages
AT mccourtpeter transcriptomeandproteomeprofilingrevealcomplementaryscavengerandimmunefeaturesofratliversinusoidalendothelialcellsandlivermacrophages
AT johansensteinardaae transcriptomeandproteomeprofilingrevealcomplementaryscavengerandimmunefeaturesofratliversinusoidalendothelialcellsandlivermacrophages
AT smedsrødbard transcriptomeandproteomeprofilingrevealcomplementaryscavengerandimmunefeaturesofratliversinusoidalendothelialcellsandlivermacrophages
AT martinezzubiaurreinigo transcriptomeandproteomeprofilingrevealcomplementaryscavengerandimmunefeaturesofratliversinusoidalendothelialcellsandlivermacrophages
AT sørensenkarenkristine transcriptomeandproteomeprofilingrevealcomplementaryscavengerandimmunefeaturesofratliversinusoidalendothelialcellsandlivermacrophages