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Opposing JAK-STAT and Wnt signaling gradients define a stem cell domain by regulating differentiation at two borders

Many adult stem cell communities are maintained by population asymmetry, where stochastic behaviors of multiple individual cells collectively result in a balance between stem cell division and differentiation. We investigated how this is achieved for Drosophila Follicle Stem Cells (FSCs) by spatiall...

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Detalles Bibliográficos
Autores principales: Melamed, David, Kalderon, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695452/
https://www.ncbi.nlm.nih.gov/pubmed/33135631
http://dx.doi.org/10.7554/eLife.61204
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author Melamed, David
Kalderon, Daniel
author_facet Melamed, David
Kalderon, Daniel
author_sort Melamed, David
collection PubMed
description Many adult stem cell communities are maintained by population asymmetry, where stochastic behaviors of multiple individual cells collectively result in a balance between stem cell division and differentiation. We investigated how this is achieved for Drosophila Follicle Stem Cells (FSCs) by spatially-restricted niche signals. FSCs produce transit-amplifying Follicle Cells (FCs) from their posterior face and quiescent Escort Cells (ECs) to their anterior. We show that JAK-STAT pathway activity, which declines from posterior to anterior, dictates the pattern of divisions over the FSC domain, promotes more posterior FSC locations and conversion to FCs, while opposing EC production. Wnt pathway activity declines from the anterior, promotes anterior FSC locations and EC production, and opposes FC production. The pathways combine to define a stem cell domain through concerted effects on FSC differentiation to ECs and FCs at either end of opposing signaling gradients, and impose a pattern of proliferation that matches derivative production.
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spelling pubmed-76954522020-11-30 Opposing JAK-STAT and Wnt signaling gradients define a stem cell domain by regulating differentiation at two borders Melamed, David Kalderon, Daniel eLife Developmental Biology Many adult stem cell communities are maintained by population asymmetry, where stochastic behaviors of multiple individual cells collectively result in a balance between stem cell division and differentiation. We investigated how this is achieved for Drosophila Follicle Stem Cells (FSCs) by spatially-restricted niche signals. FSCs produce transit-amplifying Follicle Cells (FCs) from their posterior face and quiescent Escort Cells (ECs) to their anterior. We show that JAK-STAT pathway activity, which declines from posterior to anterior, dictates the pattern of divisions over the FSC domain, promotes more posterior FSC locations and conversion to FCs, while opposing EC production. Wnt pathway activity declines from the anterior, promotes anterior FSC locations and EC production, and opposes FC production. The pathways combine to define a stem cell domain through concerted effects on FSC differentiation to ECs and FCs at either end of opposing signaling gradients, and impose a pattern of proliferation that matches derivative production. eLife Sciences Publications, Ltd 2020-11-02 /pmc/articles/PMC7695452/ /pubmed/33135631 http://dx.doi.org/10.7554/eLife.61204 Text en © 2020, Melamed and Kalderon http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Melamed, David
Kalderon, Daniel
Opposing JAK-STAT and Wnt signaling gradients define a stem cell domain by regulating differentiation at two borders
title Opposing JAK-STAT and Wnt signaling gradients define a stem cell domain by regulating differentiation at two borders
title_full Opposing JAK-STAT and Wnt signaling gradients define a stem cell domain by regulating differentiation at two borders
title_fullStr Opposing JAK-STAT and Wnt signaling gradients define a stem cell domain by regulating differentiation at two borders
title_full_unstemmed Opposing JAK-STAT and Wnt signaling gradients define a stem cell domain by regulating differentiation at two borders
title_short Opposing JAK-STAT and Wnt signaling gradients define a stem cell domain by regulating differentiation at two borders
title_sort opposing jak-stat and wnt signaling gradients define a stem cell domain by regulating differentiation at two borders
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695452/
https://www.ncbi.nlm.nih.gov/pubmed/33135631
http://dx.doi.org/10.7554/eLife.61204
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