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A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics

A bread wheat line (N11) and a disomic 2D(2R) substitution triticale line were crossed and backrossed four times. At each step electrophoretic selection for the seeds that possessed, simultaneously, the complete set of high molecular weight glutenin subunits of N11 and the two high molecular weight...

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Autores principales: Sestili, Francesco, Margiotta, Benedetta, Vaccino, Patrizia, Moscaritolo, Salvatore, Giorgi, Debora, Lucretti, Sergio, Palombieri, Samuela, Masci, Stefania, Lafiandra, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696169/
https://www.ncbi.nlm.nih.gov/pubmed/33182791
http://dx.doi.org/10.3390/ijms21228450
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author Sestili, Francesco
Margiotta, Benedetta
Vaccino, Patrizia
Moscaritolo, Salvatore
Giorgi, Debora
Lucretti, Sergio
Palombieri, Samuela
Masci, Stefania
Lafiandra, Domenico
author_facet Sestili, Francesco
Margiotta, Benedetta
Vaccino, Patrizia
Moscaritolo, Salvatore
Giorgi, Debora
Lucretti, Sergio
Palombieri, Samuela
Masci, Stefania
Lafiandra, Domenico
author_sort Sestili, Francesco
collection PubMed
description A bread wheat line (N11) and a disomic 2D(2R) substitution triticale line were crossed and backrossed four times. At each step electrophoretic selection for the seeds that possessed, simultaneously, the complete set of high molecular weight glutenin subunits of N11 and the two high molecular weight secalins of rye, present in the 2D(2R) line, was carried out. Molecular cytogenetic analyses of the BC(4)F(8) generation revealed that the selection carried out produced a disomic addition line (2n = 44). The pair of additional chromosomes consisted of the long arm of chromosome 1R (1RL) from rye fused with the satellite body of the wheat chromosome 6B. Rheological analyses revealed that the dough obtained by the new addition line had higher quality characteristics when compared with the two parents. The role of the two additional high molecular weight secalins, present in the disomic addition line, in influencing improved dough characteristics is discussed.
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spelling pubmed-76961692020-11-29 A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics Sestili, Francesco Margiotta, Benedetta Vaccino, Patrizia Moscaritolo, Salvatore Giorgi, Debora Lucretti, Sergio Palombieri, Samuela Masci, Stefania Lafiandra, Domenico Int J Mol Sci Article A bread wheat line (N11) and a disomic 2D(2R) substitution triticale line were crossed and backrossed four times. At each step electrophoretic selection for the seeds that possessed, simultaneously, the complete set of high molecular weight glutenin subunits of N11 and the two high molecular weight secalins of rye, present in the 2D(2R) line, was carried out. Molecular cytogenetic analyses of the BC(4)F(8) generation revealed that the selection carried out produced a disomic addition line (2n = 44). The pair of additional chromosomes consisted of the long arm of chromosome 1R (1RL) from rye fused with the satellite body of the wheat chromosome 6B. Rheological analyses revealed that the dough obtained by the new addition line had higher quality characteristics when compared with the two parents. The role of the two additional high molecular weight secalins, present in the disomic addition line, in influencing improved dough characteristics is discussed. MDPI 2020-11-10 /pmc/articles/PMC7696169/ /pubmed/33182791 http://dx.doi.org/10.3390/ijms21228450 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sestili, Francesco
Margiotta, Benedetta
Vaccino, Patrizia
Moscaritolo, Salvatore
Giorgi, Debora
Lucretti, Sergio
Palombieri, Samuela
Masci, Stefania
Lafiandra, Domenico
A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics
title A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics
title_full A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics
title_fullStr A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics
title_full_unstemmed A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics
title_short A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics
title_sort cross between bread wheat and a 2d(2r) disomic substitution triticale line leads to the formation of a novel disomic addition line and provides information of the role of rye secalins on breadmaking characteristics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696169/
https://www.ncbi.nlm.nih.gov/pubmed/33182791
http://dx.doi.org/10.3390/ijms21228450
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