Cargando…
A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics
A bread wheat line (N11) and a disomic 2D(2R) substitution triticale line were crossed and backrossed four times. At each step electrophoretic selection for the seeds that possessed, simultaneously, the complete set of high molecular weight glutenin subunits of N11 and the two high molecular weight...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696169/ https://www.ncbi.nlm.nih.gov/pubmed/33182791 http://dx.doi.org/10.3390/ijms21228450 |
_version_ | 1783615348156661760 |
---|---|
author | Sestili, Francesco Margiotta, Benedetta Vaccino, Patrizia Moscaritolo, Salvatore Giorgi, Debora Lucretti, Sergio Palombieri, Samuela Masci, Stefania Lafiandra, Domenico |
author_facet | Sestili, Francesco Margiotta, Benedetta Vaccino, Patrizia Moscaritolo, Salvatore Giorgi, Debora Lucretti, Sergio Palombieri, Samuela Masci, Stefania Lafiandra, Domenico |
author_sort | Sestili, Francesco |
collection | PubMed |
description | A bread wheat line (N11) and a disomic 2D(2R) substitution triticale line were crossed and backrossed four times. At each step electrophoretic selection for the seeds that possessed, simultaneously, the complete set of high molecular weight glutenin subunits of N11 and the two high molecular weight secalins of rye, present in the 2D(2R) line, was carried out. Molecular cytogenetic analyses of the BC(4)F(8) generation revealed that the selection carried out produced a disomic addition line (2n = 44). The pair of additional chromosomes consisted of the long arm of chromosome 1R (1RL) from rye fused with the satellite body of the wheat chromosome 6B. Rheological analyses revealed that the dough obtained by the new addition line had higher quality characteristics when compared with the two parents. The role of the two additional high molecular weight secalins, present in the disomic addition line, in influencing improved dough characteristics is discussed. |
format | Online Article Text |
id | pubmed-7696169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76961692020-11-29 A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics Sestili, Francesco Margiotta, Benedetta Vaccino, Patrizia Moscaritolo, Salvatore Giorgi, Debora Lucretti, Sergio Palombieri, Samuela Masci, Stefania Lafiandra, Domenico Int J Mol Sci Article A bread wheat line (N11) and a disomic 2D(2R) substitution triticale line were crossed and backrossed four times. At each step electrophoretic selection for the seeds that possessed, simultaneously, the complete set of high molecular weight glutenin subunits of N11 and the two high molecular weight secalins of rye, present in the 2D(2R) line, was carried out. Molecular cytogenetic analyses of the BC(4)F(8) generation revealed that the selection carried out produced a disomic addition line (2n = 44). The pair of additional chromosomes consisted of the long arm of chromosome 1R (1RL) from rye fused with the satellite body of the wheat chromosome 6B. Rheological analyses revealed that the dough obtained by the new addition line had higher quality characteristics when compared with the two parents. The role of the two additional high molecular weight secalins, present in the disomic addition line, in influencing improved dough characteristics is discussed. MDPI 2020-11-10 /pmc/articles/PMC7696169/ /pubmed/33182791 http://dx.doi.org/10.3390/ijms21228450 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sestili, Francesco Margiotta, Benedetta Vaccino, Patrizia Moscaritolo, Salvatore Giorgi, Debora Lucretti, Sergio Palombieri, Samuela Masci, Stefania Lafiandra, Domenico A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics |
title | A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics |
title_full | A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics |
title_fullStr | A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics |
title_full_unstemmed | A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics |
title_short | A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics |
title_sort | cross between bread wheat and a 2d(2r) disomic substitution triticale line leads to the formation of a novel disomic addition line and provides information of the role of rye secalins on breadmaking characteristics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696169/ https://www.ncbi.nlm.nih.gov/pubmed/33182791 http://dx.doi.org/10.3390/ijms21228450 |
work_keys_str_mv | AT sestilifrancesco acrossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT margiottabenedetta acrossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT vaccinopatrizia acrossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT moscaritolosalvatore acrossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT giorgidebora acrossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT lucrettisergio acrossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT palombierisamuela acrossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT mascistefania acrossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT lafiandradomenico acrossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT sestilifrancesco crossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT margiottabenedetta crossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT vaccinopatrizia crossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT moscaritolosalvatore crossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT giorgidebora crossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT lucrettisergio crossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT palombierisamuela crossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT mascistefania crossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics AT lafiandradomenico crossbetweenbreadwheatanda2d2rdisomicsubstitutiontriticalelineleadstotheformationofanoveldisomicadditionlineandprovidesinformationoftheroleofryesecalinsonbreadmakingcharacteristics |