Cargando…

Biochemical and transcript level differences between the three human phosphofructokinases show optimisation of each isoform for specific metabolic niches

6-Phosphofructokinase-1-kinase (PFK) tetramers catalyse the phosphorylation of fructose 6-phosphate (F6P) to fructose 1,6-bisphosphate (F16BP). Vertebrates have three PFK isoforms (PFK-M, PFK-L, and PFK-P). This study is the first to compare the kinetics, structures, and transcript levels of recombi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fernandes, Peter M., Kinkead, James, McNae, Iain, Michels, Paul A.M., Walkinshaw, Malcolm D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Portland Press Ltd. 2020
Materias:	Enzymology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7702303/ https://www.ncbi.nlm.nih.gov/pubmed/33141153 http://dx.doi.org/10.1042/BCJ20200656

Ejemplares similares

The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction
por: Fernandes, Peter M., et al.
Publicado: (2019)

Kinetic and structural studies of Trypanosoma and Leishmania phosphofructokinases show evolutionary divergence and identify AMP as a switch regulating glycolysis versus gluconeogenesis
por: Fernandes, Peter M., et al.
Publicado: (2020)

Fast acting allosteric phosphofructokinase inhibitors block trypanosome glycolysis and cure acute African trypanosomiasis in mice
por: McNae, Iain W., et al.
Publicado: (2021)

Comparative Kinetic and Functional Characterization of the Motor Domains of Human Nonmuscle Myosin-2C Isoforms
por: Heissler, Sarah M., et al.
Publicado: (2011)

Metabolic insights into phosphofructokinase inhibition in bloodstream-form trypanosomes
por: Nare, Zandile, et al.
Publicado: (2023)

Probing altered enzyme activity in the biochemical characterization of cancer
por: Adam, Mowaffaq Adam Ahmed, et al.
Publicado: (2022)

The Superfast Human Extraocular Myosin Is Kinetically Distinct from the Fast Skeletal IIa, IIb, and IId Isoforms
por: Bloemink, Marieke J., et al.
Publicado: (2013)

Caspases from scleractinian coral show unique regulatory features
por: Shrestha, Suman, et al.
Publicado: (2020)

Three mammalian tropomyosin isoforms have different regulatory effects on nonmuscle myosin-2B and filamentous β-actin in vitro
por: Pathan-Chhatbar, Salma, et al.
Publicado: (2018)

Ascertaining the biochemical function of an essential pectin methylesterase in the gut microbe Bacteroides thetaiotaomicron
por: Duan, Cheng-Jie, et al.
Publicado: (2021)

Biochemical and structural investigations clarify the substrate selectivity of the 2-oxoglutarate oxygenase JMJD6
por: Islam, Md. Saiful, et al.
Publicado: (2019)

Biochemical and biophysical analyses of hypoxia sensing prolyl hydroxylases from Dictyostelium discoideum and Toxoplasma gondii
por: Liu, Tongri, et al.
Publicado: (2021)

Structural and biochemical insights into the catalytic mechanisms of two insect chitin deacetylases of the carbohydrate esterase 4 family
por: Liu, Lin, et al.
Publicado: (2019)

Structural and biochemical studies of the glucuronoyl esterase OtCE15A illuminate its interaction with lignocellulosic components
por: Mazurkewich, Scott, et al.
Publicado: (2019)

GUCY2D mutations in retinal guanylyl cyclase 1 provide biochemical reasons for dominant cone–rod dystrophy but not for stationary night blindness
por: Peshenko, Igor V., et al.
Publicado: (2021)

Structures of Leishmania Fructose-1,6-Bisphosphatase Reveal Species-Specific Differences in the Mechanism of Allosteric Inhibition
por: Yuan, Meng, et al.
Publicado: (2017)

Mapping the substrate specificity of the Plasmodium M1 and M17 aminopeptidases
por: Malcolm, Tess R., et al.
Publicado: (2021)

PR Domain-containing Protein 7 (PRDM7) Is a Histone 3 Lysine 4 Trimethyltransferase
por: Blazer, Levi L., et al.
Publicado: (2016)

Trimethylation of Histone H3 Lysine 36 by Human Methyltransferase PRDM9 Protein
por: Eram, Mohammad S., et al.
Publicado: (2014)

NAD(+) biosynthesis in bacteria is controlled by global carbon/nitrogen levels via PII signaling
por: Santos, Adrian Richard Schenberger, et al.
Publicado: (2020)

Structures of pyruvate kinases display evolutionarily divergent allosteric strategies
por: Morgan, Hugh P., et al.
Publicado: (2014)

Structure and Mechanism of Human UDP-glucose 6-Dehydrogenase
por: Egger, Sigrid, et al.
Publicado: (2011)

Regulation of Response Regulator Autophosphorylation through Interdomain Contacts
por: Barbieri, Christopher M., et al.
Publicado: (2010)

Characterization of the Elongation Complex of Dengue Virus RNA Polymerase: ASSEMBLY, KINETICS OF NUCLEOTIDE INCORPORATION, AND FIDELITY
por: Jin, Zhinan, et al.
Publicado: (2011)

Molecular Characterization of a Novel Geranylgeranyl Pyrophosphate Synthase from Plasmodium Parasites
por: Artz, Jennifer D., et al.
Publicado: (2011)

The Catalytic Aspartate Is Protonated in the Michaelis Complex Formed between Trypsin and an in Vitro Evolved Substrate-like Inhibitor: A REFINED MECHANISM OF SERINE PROTEASE ACTION
por: Wahlgren, Weixiao Yuan, et al.
Publicado: (2011)

Quantitative Mass Spectrometry Reveals Dynamics of Factor-inhibiting Hypoxia-inducible Factor-catalyzed Hydroxylation
por: Singleton, Rachelle S., et al.
Publicado: (2011)

Structure and Activity of Paenibacillus polymyxa Xyloglucanase from Glycoside Hydrolase Family 44
por: Ariza, Antonio, et al.
Publicado: (2011)

Characterization of the Deoxynucleotide Triphosphate Triphosphohydrolase (dNTPase) Activity of the EF1143 Protein from Enterococcus faecalis and Crystal Structure of the Activator-Substrate Complex
por: Vorontsov, Ivan I., et al.
Publicado: (2011)

NrdH-Redoxin Protein Mediates High Enzyme Activity in Manganese-reconstituted Ribonucleotide Reductase from Bacillus anthracis
por: Crona, Mikael, et al.
Publicado: (2011)

Purification and Identification of Activating Enzymes of CS-0777, a Selective Sphingosine 1-Phosphate Receptor 1 Modulator, in Erythrocytes
por: Yonesu, Kiyoaki, et al.
Publicado: (2011)

Two Drosophila Myosin Transducer Mutants with Distinct Cardiomyopathies Have Divergent ADP and Actin Affinities
por: Bloemink, Marieke J., et al.
Publicado: (2011)

Comparative Analysis of Histophilus somni Immunoglobulin-binding Protein A (IbpA) with Other Fic Domain-containing Enzymes Reveals Differences in Substrate and Nucleotide Specificities
por: Mattoo, Seema, et al.
Publicado: (2011)

Caspase-1 Promiscuity Is Counterbalanced by Rapid Inactivation of Processed Enzyme
por: Walsh, John G., et al.
Publicado: (2011)

Mining Endonuclease Cleavage Determinants in Genomic Sequence Data
por: Szeto, Mindy D., et al.
Publicado: (2011)

Human Carboxymethylenebutenolidase as a Bioactivating Hydrolase of Olmesartan Medoxomil in Liver and Intestine
por: Ishizuka, Tomoko, et al.
Publicado: (2010)

Physical Interaction of RECQ5 Helicase with RAD51 Facilitates Its Anti-recombinase Activity
por: Schwendener, Sybille, et al.
Publicado: (2010)

Dynamic Mechanism of Proton Transfer in Mannitol 2-Dehydrogenase from Pseudomonas fluorescens: MOBILE GLU(292) CONTROLS PROTON RELAY THROUGH A WATER CHANNEL THAT CONNECTS THE ACTIVE SITE WITH BULK SOLVENT
por: Klimacek, Mario, et al.
Publicado: (2012)

Cytochrome b Mutation Y268S Conferring Atovaquone Resistance Phenotype in Malaria Parasite Results in Reduced Parasite bc(1) Catalytic Turnover and Protein Expression
por: Fisher, Nicholas, et al.
Publicado: (2012)

Assembly, Purification, and Pre-steady-state Kinetic Analysis of Active RNA-dependent RNA Polymerase Elongation Complex
por: Jin, Zhinan, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_v9b2stqpghmh3bi28n3s2ea6sa