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Protocol for efficient fluorescence 3′ end-labeling of native noncoding RNA domains

Noncoding RNAs (ncRNAs) comprise a class of versatile transcripts that are highly involved in the regulation of a wide range of biological processes. Functional long ncRNAs (> 200 nts in length) often adopt secondary structures that arise co-transcriptionally. To maintain the secondary structure...

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Detalles Bibliográficos
Autores principales: Awwad, Dahlia A., Rahmouni, A. Rachid, Aboul-ela, Fareed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7704408/
https://www.ncbi.nlm.nih.gov/pubmed/33299805
http://dx.doi.org/10.1016/j.mex.2020.101148