Propidium monoazide pretreatment on a 3D-printed microfluidic device for efficient PCR determination of live versus dead’microbial cells†

Waterborne microbial pathogen detection via nucleic acid analysis on portable microfluidic devices is a growing area of research, development, and application. Traditional polymerase chain reaction (PCR)-based nucleic acid analysis detects total extracted DNA, but cannot differentiate live and dead...

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Detalles Bibliográficos
Autores principales: Zhu, Yanzhe, Huang, Xiao, Xie, Xing, Bahnemann, Janina, Lin, Xingyu, Wu, Xunyi, Wang, Siwen, Hoffmann, Michael R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705123/
https://www.ncbi.nlm.nih.gov/pubmed/33365136
http://dx.doi.org/10.1039/c8ew00058a

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705123/
https://www.ncbi.nlm.nih.gov/pubmed/33365136
http://dx.doi.org/10.1039/c8ew00058a

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