A robust CRISPR–Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair

DNA double-strand breaks (DSBs) are highly cytotoxic lesions that can lead to chromosome rearrangements, genomic instability and cell death. Consequently, cells have evolved multiple mechanisms to efficiently repair DSBs to preserve genomic integrity. We have developed a DSB repair assay system, des...

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Detalles Bibliográficos
Autores principales: Eki, Rebeka, She, Jane, Parlak, Mahmut, Benamar, Mouadh, Du, Kang-Ping, Kumar, Pankaj, Abbas, Tarek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708081/
https://www.ncbi.nlm.nih.gov/pubmed/33068408
http://dx.doi.org/10.1093/nar/gkaa897

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708081/
https://www.ncbi.nlm.nih.gov/pubmed/33068408
http://dx.doi.org/10.1093/nar/gkaa897

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