An efficient and precise method for generating knockout cell lines based on CRISPR‐Cas9 system

Although the efficiency and versatility of CRISPR‐Cas9 system has been greatly improved over conventional genome editing methods such as zinc finger or TALEN, it is still time‐consuming and labor‐intensive for screening knockout/knock‐in cell clones due to differences of the targeted location or eff...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Xibin, Guo, Yuhan, Gu, Shu, Tan, Deng, Cheng, Baoyun, Li, Zhoufang, Huang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708952/
https://www.ncbi.nlm.nih.gov/pubmed/33304232
http://dx.doi.org/10.1002/elsc.202000032

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708952/
https://www.ncbi.nlm.nih.gov/pubmed/33304232
http://dx.doi.org/10.1002/elsc.202000032

Ejemplares similares