The copper(II)-binding tripeptide GHK, a valuable crystallization and phasing tag for macromolecular crystallography

The growth of diffraction-quality crystals and experimental phasing remain two of the main bottlenecks in protein crystallography. Here, the high-affinity copper(II)-binding tripeptide GHK was fused to the N-terminus of a GFP variant and an MBP-FG peptide fusion. The GHK tag promoted crystallization...

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Detalles Bibliográficos
Autores principales: Mehr, Alexander, Henneberg, Fabian, Chari, Ashwin, Görlich, Dirk, Huyton, Trevor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709198/
https://www.ncbi.nlm.nih.gov/pubmed/33263328
http://dx.doi.org/10.1107/S2059798320013741

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709198/
https://www.ncbi.nlm.nih.gov/pubmed/33263328
http://dx.doi.org/10.1107/S2059798320013741

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