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The copper(II)-binding tripeptide GHK, a valuable crystallization and phasing tag for macromolecular crystallography
The growth of diffraction-quality crystals and experimental phasing remain two of the main bottlenecks in protein crystallography. Here, the high-affinity copper(II)-binding tripeptide GHK was fused to the N-terminus of a GFP variant and an MBP-FG peptide fusion. The GHK tag promoted crystallization...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
International Union of Crystallography
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709198/ https://www.ncbi.nlm.nih.gov/pubmed/33263328 http://dx.doi.org/10.1107/S2059798320013741 |