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Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

Electron crystallography of sub-micrometre-sized 3D protein crystals has emerged recently as a valuable field of structural biology. In meso crystallization methods, utilizing lipidic mesophases, particularly lipidic cubic phases (LCPs), can produce high-quality 3D crystals of membrane proteins (MPs...

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Detalles Bibliográficos
Autores principales: Polovinkin, Vitaly, Khakurel, Krishna, Babiak, Michal, Angelov, Borislav, Schneider, Bohdan, Dohnalek, Jan, Andreasson, Jakob, Hajdu, Janos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710488/
https://www.ncbi.nlm.nih.gov/pubmed/33304220
http://dx.doi.org/10.1107/S1600576720013096