A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells

Melanoma is an aggressive skin tumor that shows a high mortality rate and level of metastasis. BRAF gene mutation (BRAF V600E) is directly related to the occurrence of melanoma. In this study, a light-inducible gene expression system was designed to control the Cas9 transcription, which could then c...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Xia, Huang, Haiyan, Yu, Bo, Zhang, Jianzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710612/
https://www.ncbi.nlm.nih.gov/pubmed/33330635
http://dx.doi.org/10.3389/fmolb.2020.606593
_version_ 1783617973502607360
author Wu, Xia
Huang, Haiyan
Yu, Bo
Zhang, Jianzhong
author_facet Wu, Xia
Huang, Haiyan
Yu, Bo
Zhang, Jianzhong
author_sort Wu, Xia
collection PubMed
description Melanoma is an aggressive skin tumor that shows a high mortality rate and level of metastasis. BRAF gene mutation (BRAF V600E) is directly related to the occurrence of melanoma. In this study, a light-inducible gene expression system was designed to control the Cas9 transcription, which could then cleave the BRAF V600E. To prove the potential utility of this system in melanoma, the physiological function of melanoma cells was tested. It illustrated that the light-induced CRISPR-Cas9 system could inhibit the progression of G361 and A375 cells. Thus, this system may provide a novel therapeutic strategy of melanoma intervention.
format Online
Article
Text
id pubmed-7710612
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-77106122020-12-15 A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells Wu, Xia Huang, Haiyan Yu, Bo Zhang, Jianzhong Front Mol Biosci Molecular Biosciences Melanoma is an aggressive skin tumor that shows a high mortality rate and level of metastasis. BRAF gene mutation (BRAF V600E) is directly related to the occurrence of melanoma. In this study, a light-inducible gene expression system was designed to control the Cas9 transcription, which could then cleave the BRAF V600E. To prove the potential utility of this system in melanoma, the physiological function of melanoma cells was tested. It illustrated that the light-induced CRISPR-Cas9 system could inhibit the progression of G361 and A375 cells. Thus, this system may provide a novel therapeutic strategy of melanoma intervention. Frontiers Media S.A. 2020-11-19 /pmc/articles/PMC7710612/ /pubmed/33330635 http://dx.doi.org/10.3389/fmolb.2020.606593 Text en Copyright © 2020 Wu, Huang, Yu and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Wu, Xia
Huang, Haiyan
Yu, Bo
Zhang, Jianzhong
A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_full A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_fullStr A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_full_unstemmed A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_short A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_sort blue light-inducible crispr-cas9 system for inhibiting progression of melanoma cells
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710612/
https://www.ncbi.nlm.nih.gov/pubmed/33330635
http://dx.doi.org/10.3389/fmolb.2020.606593
work_keys_str_mv AT wuxia abluelightinduciblecrisprcas9systemforinhibitingprogressionofmelanomacells
AT huanghaiyan abluelightinduciblecrisprcas9systemforinhibitingprogressionofmelanomacells
AT yubo abluelightinduciblecrisprcas9systemforinhibitingprogressionofmelanomacells
AT zhangjianzhong abluelightinduciblecrisprcas9systemforinhibitingprogressionofmelanomacells
AT wuxia bluelightinduciblecrisprcas9systemforinhibitingprogressionofmelanomacells
AT huanghaiyan bluelightinduciblecrisprcas9systemforinhibitingprogressionofmelanomacells
AT yubo bluelightinduciblecrisprcas9systemforinhibitingprogressionofmelanomacells
AT zhangjianzhong bluelightinduciblecrisprcas9systemforinhibitingprogressionofmelanomacells

Ejemplares similares