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Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation
Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct insight into cell metabolism, cell dynamics and protein activity. However, determining the lifetimes of different fluorescent proteins requires the detection of a relatively large number of photons, hence slowin...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710711/ https://www.ncbi.nlm.nih.gov/pubmed/33268900 http://dx.doi.org/10.1038/s41598-020-77737-0 |