An Efficient Method for Isolation of Plasmid DNA for Transfection of Mammalian Cell Cultures

In this article, we present several protocols that describe the steps from cloning and obtaining a large amount of pure plasmid DNA to generation of lentiviruses based on these constructs. The protocols have been worked out on human cell culture HEK293T but can be adapted for other cell cultures. Th...

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Detalles Bibliográficos
Autores principales: Kachkin, Daniel V., Khorolskaya, Julia I., Ivanova, Julia S., Rubel, Aleksandr A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712542/
https://www.ncbi.nlm.nih.gov/pubmed/33066602
http://dx.doi.org/10.3390/mps3040069