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Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression System

The limited amount of fusion protein transported into cytosol milieu has made it challenging to obtain a sufficient amount for further applications. To avoid the laborious and expensive task, T7 promoter-driving pET-30a(+) coding for chimeric gene of thymidine phosphorylase and core streptavidin as...

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Detalles Bibliográficos
Autores principales: Tarar, Ammar, Alyami, Esmael M., Peng, Ching-An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712975/
https://www.ncbi.nlm.nih.gov/pubmed/33271819
http://dx.doi.org/10.3390/mps3040082