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A novel reverse two-hybrid method for the identification of missense mutations that disrupt protein–protein binding
The reverse two-hybrid system is a powerful method to select mutations that disrupt the interaction between two proteins and therefore to identify the residues involved in this interaction. However, the usefulness of this technique has been limited by its relative complexity when compared to the cla...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713115/ https://www.ncbi.nlm.nih.gov/pubmed/33273586 http://dx.doi.org/10.1038/s41598-020-77992-1 |