Improving CRISPR/Cas9 mutagenesis efficiency by delaying the early development of zebrafish embryos

CRISPR/Cas9 driven mutagenesis in zygotes is a popular tool for introducing targeted mutations in model organisms. Compared to mouse, mutagenesis in zebrafish is relatively inefficient and results in somatic mosaicism most likely due to a short single-cell stage of about 40 min. Here we explored two...

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Detalles Bibliográficos
Autores principales: Terzioglu, M., Saralahti, A., Piippo, H., Rämet, M., Andressoo, J.-O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713128/
https://www.ncbi.nlm.nih.gov/pubmed/33273577
http://dx.doi.org/10.1038/s41598-020-77677-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713128/
https://www.ncbi.nlm.nih.gov/pubmed/33273577
http://dx.doi.org/10.1038/s41598-020-77677-9

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