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Improving CRISPR/Cas9 mutagenesis efficiency by delaying the early development of zebrafish embryos
CRISPR/Cas9 driven mutagenesis in zygotes is a popular tool for introducing targeted mutations in model organisms. Compared to mouse, mutagenesis in zebrafish is relatively inefficient and results in somatic mosaicism most likely due to a short single-cell stage of about 40 min. Here we explored two...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713128/ https://www.ncbi.nlm.nih.gov/pubmed/33273577 http://dx.doi.org/10.1038/s41598-020-77677-9 |