Cargando…
Mechanistic studies of gene delivery into mammalian cells by electrical short-circuiting via an aqueous droplet in dielectric oil
We have developed a novel methodology for the delivery of cell-impermeable molecules, based on electrical short-circuiting via a water droplet in dielectric oil. When a cell suspension droplet is placed between a pair of electrodes with an intense DC electric field, droplet bouncing and droplet defo...
Autores principales: | Kurita, Hirofumi, Nihonyanagi, Hirohito, Watanabe, Yuki, Sugano, Kenta, Shinozaki, Ryuto, Kishikawa, Kenta, Numano, Rika, Takashima, Kazunori |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717561/ https://www.ncbi.nlm.nih.gov/pubmed/33275626 http://dx.doi.org/10.1371/journal.pone.0243361 |
Ejemplares similares
-
Influence of Electroporation Medium on Delivery of Cell-Impermeable Small Molecules by Electrical Short-Circuiting via an Aqueous Droplet in Dielectric Oil: A Comparison of Different Fluorescent Tracers
por: Watanabe, Yuki, et al.
Publicado: (2022) -
Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field
por: Kurita, Hirofumi, et al.
Publicado: (2016) -
Influence of DNA characteristics on cell membrane damage stimulated by electrical short-circuiting via a low-conductive aqueous droplet in dielectric oil
por: Tsurusaki, Yoshino, et al.
Publicado: (2023) -
Novel Parallelized Electroporation by Electrostatic Manipulation of a Water-in-Oil Droplet as a Microreactor
por: Kurita, Hirofumi, et al.
Publicado: (2015) -
Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation
por: ISHINO, Takeshi, et al.
Publicado: (2019)