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3D projection electrophoresis for single-cell immunoblotting
Immunoassays and mass spectrometry are powerful single-cell protein analysis tools; however, interfacing and throughput bottlenecks remain. Here, we introduce three-dimensional single-cell immunoblots to detect both cytosolic and nuclear proteins. The 3D microfluidic device is a photoactive polyacry...
Autores principales: | Grist, Samantha M., Mourdoukoutas, Andoni P., Herr, Amy E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718224/ https://www.ncbi.nlm.nih.gov/pubmed/33277486 http://dx.doi.org/10.1038/s41467-020-19738-1 |
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