Production of isoform-specific knockdown/knockout Madin–Darby canine kidney epithelial cells using CRISPR/Cas9

CRISPR-Cas9 gene editing has made it possible to specifically edit genes in a myriad of target cells. Here, a method for isoform-specific editing and clonal selection in Madin-Darby canine kidney (MDCK) epithelial cells is described in detail. This approach was used to address a long-standing questi...

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Detalles Bibliográficos
Autores principales: Readler, James M., AlKahlout, Amal S., Kolawole, Abimbola O., Excoffon, Katherine J.D.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718483/
https://www.ncbi.nlm.nih.gov/pubmed/33304833
http://dx.doi.org/10.1016/j.mex.2020.101149

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718483/
https://www.ncbi.nlm.nih.gov/pubmed/33304833
http://dx.doi.org/10.1016/j.mex.2020.101149

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