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Preventing Photomorbidity in Long-Term Multi-color Fluorescence Imaging of Saccharomyces cerevisiae and S. pombe

Time-lapse imaging of live cells using multiple fluorescent reporters is an essential tool to study molecular processes in single cells. However, exposure to even moderate doses of visible excitation light can disturb cellular physiology and alter the quantitative behavior of the cells under study....

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Detalles Bibliográficos
Autores principales: Schmidt, Gregor W., Cuny, Andreas P., Rudolf, Fabian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718758/
https://www.ncbi.nlm.nih.gov/pubmed/33023973
http://dx.doi.org/10.1534/g3.120.401465