Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1

Accurate yet efficient high-throughput screenings have emerged as essential technology for enzyme engineering via directed evolution. Modern high-throughput screening platforms for oxidoreductases are commonly assisted by technologies such as surface display and rely on emulsification techniques to...

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Detalles Bibliográficos
Autores principales: Herzog, P L, Borghi, E, Traxlmayr, M W, Obinger, C, Sikes, H D, Peterbauer, C K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720637/
https://www.ncbi.nlm.nih.gov/pubmed/32930800
http://dx.doi.org/10.1093/protein/gzaa019

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720637/
https://www.ncbi.nlm.nih.gov/pubmed/32930800
http://dx.doi.org/10.1093/protein/gzaa019

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