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Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1
Accurate yet efficient high-throughput screenings have emerged as essential technology for enzyme engineering via directed evolution. Modern high-throughput screening platforms for oxidoreductases are commonly assisted by technologies such as surface display and rely on emulsification techniques to...
Autores principales: | Herzog, P L, Borghi, E, Traxlmayr, M W, Obinger, C, Sikes, H D, Peterbauer, C K |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720637/ https://www.ncbi.nlm.nih.gov/pubmed/32930800 http://dx.doi.org/10.1093/protein/gzaa019 |
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