SpEDIT: A fast and efficient CRISPR/Cas9 method for fission yeast

The CRISPR/Cas9 system allows scarless, marker-free genome editing. Current CRISPR/Cas9 systems for the fission yeast  Schizosaccharomyces pombe rely on tedious and time-consuming cloning procedures to introduce a specific sgRNA target sequence into a Cas9-expressing plasmid. In addition, Cas9 endon...

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Detalles Bibliográficos
Autores principales: Torres-Garcia, Sito, Di Pompeo, Lorenza, Eivers, Luke, Gaborieau, Baptiste, White, Sharon A., Pidoux, Alison L., Kanigowska, Paulina, Yaseen, Imtiyaz, Cai, Yizhi, Allshire, Robin C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2020
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721064/
https://www.ncbi.nlm.nih.gov/pubmed/33313420
http://dx.doi.org/10.12688/wellcomeopenres.16405.1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721064/
https://www.ncbi.nlm.nih.gov/pubmed/33313420
http://dx.doi.org/10.12688/wellcomeopenres.16405.1

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