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Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes

This study investigated whether dietary metabolizable energy (ME) could generate dynamical effects on rumen fermentation, gastrointestinal tract (GIT) morphology, and microbial composition of growing ewes. A total of twenty‐eight female Hu lambs were randomly allotted to two treatments with differen...

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Autores principales: Wang, Qiye, Wang, Yancan, Wang, Xin, Dai, Chunpeng, Tang, Wensheng, Li, Jianzhong, Huang, Pengfei, Li, Yali, Ding, Xueqin, Huang, Jing, Hussain, Tarique, Yang, Huansheng, Zhu, Mingzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723210/
https://www.ncbi.nlm.nih.gov/pubmed/33312546
http://dx.doi.org/10.1002/fsn3.1955
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author Wang, Qiye
Wang, Yancan
Wang, Xin
Dai, Chunpeng
Tang, Wensheng
Li, Jianzhong
Huang, Pengfei
Li, Yali
Ding, Xueqin
Huang, Jing
Hussain, Tarique
Yang, Huansheng
Zhu, Mingzhi
author_facet Wang, Qiye
Wang, Yancan
Wang, Xin
Dai, Chunpeng
Tang, Wensheng
Li, Jianzhong
Huang, Pengfei
Li, Yali
Ding, Xueqin
Huang, Jing
Hussain, Tarique
Yang, Huansheng
Zhu, Mingzhi
author_sort Wang, Qiye
collection PubMed
description This study investigated whether dietary metabolizable energy (ME) could generate dynamical effects on rumen fermentation, gastrointestinal tract (GIT) morphology, and microbial composition of growing ewes. A total of twenty‐eight female Hu lambs were randomly allotted to two treatments with different dietary ME levels: 9.17 (FEA) and 10.41 MJ/kg (FEB). These lambs were further made ready for a 67‐day feeding trial. Results showed that the molar proportions of butyrate (p = .020), iso‐valerate (p = .028), and valerate (p = .005) were significantly higher in the FEB group than those in the FEA group. The results of the GIT morphologic properties showed that the villus height (VH) (p = .005) was significantly higher and crypt depth was significantly deeper (CD) (p = .005) in the duodenum and that the rumen papillary height (PH) was significantly higher (p = .020) in FEB group compared with the FEA group. High‐throughput sequencing results showed that 1826 operational taxonomic units (OTUs) were obtained and that the OTU number (p = .039), the ACE (p = .035), and Chao1 indices (p = .005) were lower in the FEB group. Moreover, 76 genera belonging to 21 phyla were detected in all samples; the relative abundance of Papillibacter (p = .036) and Flexilinea (p = .046) was significantly lower in the high energy group, whereas the relative abundance of unidentified Lachnospiraceae (p = .019), Acetitomaculum (p = .029), unidentified Veillonellaceae (p = .017), Anaerovibrio (p = .005), and Succinivibrio (p = .035) was significantly higher in the FEB group at the genus level. Furthermore, the relative abundance of genes and metabolic pathways were predicted by PICRUSt. The relative abundance of gene families related to carbohydrate metabolism was particularly higher (p = .027) in the FEB group. In summary, these results reveal that the dietary energy levels altered the composition and function of rumen microbiota and GIT morphology in growing female Hu sheep and provide a reference for optimizing diet formula and 10.41MJ/kg of ME level has been recommended in the growing period.
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spelling pubmed-77232102020-12-11 Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes Wang, Qiye Wang, Yancan Wang, Xin Dai, Chunpeng Tang, Wensheng Li, Jianzhong Huang, Pengfei Li, Yali Ding, Xueqin Huang, Jing Hussain, Tarique Yang, Huansheng Zhu, Mingzhi Food Sci Nutr Original Research This study investigated whether dietary metabolizable energy (ME) could generate dynamical effects on rumen fermentation, gastrointestinal tract (GIT) morphology, and microbial composition of growing ewes. A total of twenty‐eight female Hu lambs were randomly allotted to two treatments with different dietary ME levels: 9.17 (FEA) and 10.41 MJ/kg (FEB). These lambs were further made ready for a 67‐day feeding trial. Results showed that the molar proportions of butyrate (p = .020), iso‐valerate (p = .028), and valerate (p = .005) were significantly higher in the FEB group than those in the FEA group. The results of the GIT morphologic properties showed that the villus height (VH) (p = .005) was significantly higher and crypt depth was significantly deeper (CD) (p = .005) in the duodenum and that the rumen papillary height (PH) was significantly higher (p = .020) in FEB group compared with the FEA group. High‐throughput sequencing results showed that 1826 operational taxonomic units (OTUs) were obtained and that the OTU number (p = .039), the ACE (p = .035), and Chao1 indices (p = .005) were lower in the FEB group. Moreover, 76 genera belonging to 21 phyla were detected in all samples; the relative abundance of Papillibacter (p = .036) and Flexilinea (p = .046) was significantly lower in the high energy group, whereas the relative abundance of unidentified Lachnospiraceae (p = .019), Acetitomaculum (p = .029), unidentified Veillonellaceae (p = .017), Anaerovibrio (p = .005), and Succinivibrio (p = .035) was significantly higher in the FEB group at the genus level. Furthermore, the relative abundance of genes and metabolic pathways were predicted by PICRUSt. The relative abundance of gene families related to carbohydrate metabolism was particularly higher (p = .027) in the FEB group. In summary, these results reveal that the dietary energy levels altered the composition and function of rumen microbiota and GIT morphology in growing female Hu sheep and provide a reference for optimizing diet formula and 10.41MJ/kg of ME level has been recommended in the growing period. John Wiley and Sons Inc. 2020-11-10 /pmc/articles/PMC7723210/ /pubmed/33312546 http://dx.doi.org/10.1002/fsn3.1955 Text en © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Wang, Qiye
Wang, Yancan
Wang, Xin
Dai, Chunpeng
Tang, Wensheng
Li, Jianzhong
Huang, Pengfei
Li, Yali
Ding, Xueqin
Huang, Jing
Hussain, Tarique
Yang, Huansheng
Zhu, Mingzhi
Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes
title Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes
title_full Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes
title_fullStr Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes
title_full_unstemmed Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes
title_short Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes
title_sort effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723210/
https://www.ncbi.nlm.nih.gov/pubmed/33312546
http://dx.doi.org/10.1002/fsn3.1955
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