Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis

Using a covalent chemical probe and X-ray crystallography coupled to nuclear magnetic resonance data, we elucidated the dynamic molecular basis of protein recognition between the carrier protein and adenylation domain in pyoluteorin biosynthesis. These findings reveal a unique binding mode, which co...

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Detalles Bibliográficos
Autores principales: Corpuz, Joshua C., Podust, Larissa M., Davis, Tony D., Jaremko, Matt J., Burkart, Michael D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723355/
https://www.ncbi.nlm.nih.gov/pubmed/33305272
http://dx.doi.org/10.1039/c9cb00015a
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author Corpuz, Joshua C.
Podust, Larissa M.
Davis, Tony D.
Jaremko, Matt J.
Burkart, Michael D.
author_facet Corpuz, Joshua C.
Podust, Larissa M.
Davis, Tony D.
Jaremko, Matt J.
Burkart, Michael D.
author_sort Corpuz, Joshua C.
collection PubMed
description Using a covalent chemical probe and X-ray crystallography coupled to nuclear magnetic resonance data, we elucidated the dynamic molecular basis of protein recognition between the carrier protein and adenylation domain in pyoluteorin biosynthesis. These findings reveal a unique binding mode, which contrasts previously solved carrier protein and partner protein interfaces.
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spelling pubmed-77233552021-04-01 Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis Corpuz, Joshua C. Podust, Larissa M. Davis, Tony D. Jaremko, Matt J. Burkart, Michael D. RSC Chem Biol Chemistry Using a covalent chemical probe and X-ray crystallography coupled to nuclear magnetic resonance data, we elucidated the dynamic molecular basis of protein recognition between the carrier protein and adenylation domain in pyoluteorin biosynthesis. These findings reveal a unique binding mode, which contrasts previously solved carrier protein and partner protein interfaces. RSC 2020-03-24 /pmc/articles/PMC7723355/ /pubmed/33305272 http://dx.doi.org/10.1039/c9cb00015a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Corpuz, Joshua C.
Podust, Larissa M.
Davis, Tony D.
Jaremko, Matt J.
Burkart, Michael D.
Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis
title Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis
title_full Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis
title_fullStr Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis
title_full_unstemmed Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis
title_short Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis
title_sort dynamic visualization of type ii peptidyl carrier protein recognition in pyoluteorin biosynthesis
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723355/
https://www.ncbi.nlm.nih.gov/pubmed/33305272
http://dx.doi.org/10.1039/c9cb00015a
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