Cargando…

Carvacrol Targets SarA and CrtM of Methicillin-Resistant Staphylococcus aureus to Mitigate Biofilm Formation and Staphyloxanthin Synthesis: An In Vitro and In Vivo Approach

[Image: see text] Carvacrol is an essential oil traditionally used in culinary processes as spice due to its aromatic nature and also known for various biological activities. In the present study, the antivirulence efficacy of carvacrol against methicillin-resistant Staphylococcus aureus (MRSA) is e...

Descripción completa

Detalles Bibliográficos
Autores principales: Selvaraj, Anthonymuthu, Valliammai, Alaguvel, Muthuramalingam, Pandiyan, Priya, Arumugam, Suba, Manokaran, Ramesh, Manikandan, Karutha Pandian, Shunmugiah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726784/
https://www.ncbi.nlm.nih.gov/pubmed/33324819
http://dx.doi.org/10.1021/acsomega.0c04252
_version_ 1783620954402848768
author Selvaraj, Anthonymuthu
Valliammai, Alaguvel
Muthuramalingam, Pandiyan
Priya, Arumugam
Suba, Manokaran
Ramesh, Manikandan
Karutha Pandian, Shunmugiah
author_facet Selvaraj, Anthonymuthu
Valliammai, Alaguvel
Muthuramalingam, Pandiyan
Priya, Arumugam
Suba, Manokaran
Ramesh, Manikandan
Karutha Pandian, Shunmugiah
author_sort Selvaraj, Anthonymuthu
collection PubMed
description [Image: see text] Carvacrol is an essential oil traditionally used in culinary processes as spice due to its aromatic nature and also known for various biological activities. In the present study, the antivirulence efficacy of carvacrol against methicillin-resistant Staphylococcus aureus (MRSA) is explored. MRSA is an opportunistic pathogen capable of causing various superficial and systemic infections in humans. Biofilm formation and virulence factors of MRSA are responsible for its pathogenesis and resistance. Hence, the aim of this study was to explore the antibiofilm and antivirulence efficacy of carvacrol against MRSA. Carvacrol at 75 μg/mL inhibited MRSA biofilm by 93%, and it also decreased the biofilm formation on polystyrene and glass surfaces. Further, microscopic analyses revealed the reduction in microcolony formation and collapsed structure of biofilm upon carvacrol treatment. The growth curve analysis and the Alamar blue assay showed the nonfatal effect of carvacrol on MRSA. Further, carvacrol significantly reduced the production of MRSA biofilm-associated slime and extracellular polysaccharide. In addition, carvacrol strongly inhibited the antioxidant pigment staphyloxanthin and its intermediates’ synthesis in MRSA. Inhibition of biofilm and staphyloxanthin by carvacrol enhanced the susceptibility of MRSA to oxidants and healthy human blood. Quantitative polymerase chain reaction (qPCR) analysis unveiled the downregulation of sarA-mediated biofilm gene expression and staphyloxanthin-associated crtM gene expression. The sarA-dependent antibiofilm potential of carvacrol was validated using S. aureus Newman wild-type and isogenic ΔsarA strains. In silico molecular docking analysis showed the high binding efficacy of carvacrol with staphylococcal accessory regulator A (SarA) and 4,4′-diapophytoene synthase (CrtM) when compared to positive controls. Furthermore, the in vivo efficacy of carvacrol against MRSA infection was demonstrated using the model organism Galleria mellonella. The results revealed the nontoxic nature of carvacrol to the larvae and the rescuing potential of carvacrol against MRSA infection. Finally, the current study reveals the potential of carvacrol in inhibiting the biofilm formation and staphyloxanthin synthesis of MRSA by targeting the global regulator SarA and a novel antivirulence target CrtM.
format Online
Article
Text
id pubmed-7726784
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-77267842020-12-14 Carvacrol Targets SarA and CrtM of Methicillin-Resistant Staphylococcus aureus to Mitigate Biofilm Formation and Staphyloxanthin Synthesis: An In Vitro and In Vivo Approach Selvaraj, Anthonymuthu Valliammai, Alaguvel Muthuramalingam, Pandiyan Priya, Arumugam Suba, Manokaran Ramesh, Manikandan Karutha Pandian, Shunmugiah ACS Omega [Image: see text] Carvacrol is an essential oil traditionally used in culinary processes as spice due to its aromatic nature and also known for various biological activities. In the present study, the antivirulence efficacy of carvacrol against methicillin-resistant Staphylococcus aureus (MRSA) is explored. MRSA is an opportunistic pathogen capable of causing various superficial and systemic infections in humans. Biofilm formation and virulence factors of MRSA are responsible for its pathogenesis and resistance. Hence, the aim of this study was to explore the antibiofilm and antivirulence efficacy of carvacrol against MRSA. Carvacrol at 75 μg/mL inhibited MRSA biofilm by 93%, and it also decreased the biofilm formation on polystyrene and glass surfaces. Further, microscopic analyses revealed the reduction in microcolony formation and collapsed structure of biofilm upon carvacrol treatment. The growth curve analysis and the Alamar blue assay showed the nonfatal effect of carvacrol on MRSA. Further, carvacrol significantly reduced the production of MRSA biofilm-associated slime and extracellular polysaccharide. In addition, carvacrol strongly inhibited the antioxidant pigment staphyloxanthin and its intermediates’ synthesis in MRSA. Inhibition of biofilm and staphyloxanthin by carvacrol enhanced the susceptibility of MRSA to oxidants and healthy human blood. Quantitative polymerase chain reaction (qPCR) analysis unveiled the downregulation of sarA-mediated biofilm gene expression and staphyloxanthin-associated crtM gene expression. The sarA-dependent antibiofilm potential of carvacrol was validated using S. aureus Newman wild-type and isogenic ΔsarA strains. In silico molecular docking analysis showed the high binding efficacy of carvacrol with staphylococcal accessory regulator A (SarA) and 4,4′-diapophytoene synthase (CrtM) when compared to positive controls. Furthermore, the in vivo efficacy of carvacrol against MRSA infection was demonstrated using the model organism Galleria mellonella. The results revealed the nontoxic nature of carvacrol to the larvae and the rescuing potential of carvacrol against MRSA infection. Finally, the current study reveals the potential of carvacrol in inhibiting the biofilm formation and staphyloxanthin synthesis of MRSA by targeting the global regulator SarA and a novel antivirulence target CrtM. American Chemical Society 2020-11-24 /pmc/articles/PMC7726784/ /pubmed/33324819 http://dx.doi.org/10.1021/acsomega.0c04252 Text en © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Selvaraj, Anthonymuthu
Valliammai, Alaguvel
Muthuramalingam, Pandiyan
Priya, Arumugam
Suba, Manokaran
Ramesh, Manikandan
Karutha Pandian, Shunmugiah
Carvacrol Targets SarA and CrtM of Methicillin-Resistant Staphylococcus aureus to Mitigate Biofilm Formation and Staphyloxanthin Synthesis: An In Vitro and In Vivo Approach
title Carvacrol Targets SarA and CrtM of Methicillin-Resistant Staphylococcus aureus to Mitigate Biofilm Formation and Staphyloxanthin Synthesis: An In Vitro and In Vivo Approach
title_full Carvacrol Targets SarA and CrtM of Methicillin-Resistant Staphylococcus aureus to Mitigate Biofilm Formation and Staphyloxanthin Synthesis: An In Vitro and In Vivo Approach
title_fullStr Carvacrol Targets SarA and CrtM of Methicillin-Resistant Staphylococcus aureus to Mitigate Biofilm Formation and Staphyloxanthin Synthesis: An In Vitro and In Vivo Approach
title_full_unstemmed Carvacrol Targets SarA and CrtM of Methicillin-Resistant Staphylococcus aureus to Mitigate Biofilm Formation and Staphyloxanthin Synthesis: An In Vitro and In Vivo Approach
title_short Carvacrol Targets SarA and CrtM of Methicillin-Resistant Staphylococcus aureus to Mitigate Biofilm Formation and Staphyloxanthin Synthesis: An In Vitro and In Vivo Approach
title_sort carvacrol targets sara and crtm of methicillin-resistant staphylococcus aureus to mitigate biofilm formation and staphyloxanthin synthesis: an in vitro and in vivo approach
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726784/
https://www.ncbi.nlm.nih.gov/pubmed/33324819
http://dx.doi.org/10.1021/acsomega.0c04252
work_keys_str_mv AT selvarajanthonymuthu carvacroltargetssaraandcrtmofmethicillinresistantstaphylococcusaureustomitigatebiofilmformationandstaphyloxanthinsynthesisaninvitroandinvivoapproach
AT valliammaialaguvel carvacroltargetssaraandcrtmofmethicillinresistantstaphylococcusaureustomitigatebiofilmformationandstaphyloxanthinsynthesisaninvitroandinvivoapproach
AT muthuramalingampandiyan carvacroltargetssaraandcrtmofmethicillinresistantstaphylococcusaureustomitigatebiofilmformationandstaphyloxanthinsynthesisaninvitroandinvivoapproach
AT priyaarumugam carvacroltargetssaraandcrtmofmethicillinresistantstaphylococcusaureustomitigatebiofilmformationandstaphyloxanthinsynthesisaninvitroandinvivoapproach
AT subamanokaran carvacroltargetssaraandcrtmofmethicillinresistantstaphylococcusaureustomitigatebiofilmformationandstaphyloxanthinsynthesisaninvitroandinvivoapproach
AT rameshmanikandan carvacroltargetssaraandcrtmofmethicillinresistantstaphylococcusaureustomitigatebiofilmformationandstaphyloxanthinsynthesisaninvitroandinvivoapproach
AT karuthapandianshunmugiah carvacroltargetssaraandcrtmofmethicillinresistantstaphylococcusaureustomitigatebiofilmformationandstaphyloxanthinsynthesisaninvitroandinvivoapproach