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Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay

The consumption of milk and unpasteurized dairy products contaminated with Brucella bacteria is one of the most important ways of brucellosis transmission to humans. The principal goal of this study was to determine the prevalence of Brucella abortus (B. abortus) and Brucella melitens (B. melitens)...

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Autores principales: Abdali, Fargol, Hosseinzadeh, Saeid, Berizi, Enayat, Pourmontaseri, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727767/
https://www.ncbi.nlm.nih.gov/pubmed/33313331
http://dx.doi.org/10.22099/mbrc.2020.37381.1506
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author Abdali, Fargol
Hosseinzadeh, Saeid
Berizi, Enayat
Pourmontaseri, Maryam
author_facet Abdali, Fargol
Hosseinzadeh, Saeid
Berizi, Enayat
Pourmontaseri, Maryam
author_sort Abdali, Fargol
collection PubMed
description The consumption of milk and unpasteurized dairy products contaminated with Brucella bacteria is one of the most important ways of brucellosis transmission to humans. The principal goal of this study was to determine the prevalence of Brucella abortus (B. abortus) and Brucella melitens (B. melitens) in unpasteurized dairy products consumed in Shiraz province. In this study conducted in 2016, 238 unpasteurized dairy products including 48 raw milk, 48 yogurt, 46 cheeses, 48 dough and 48 ice cream samples, were purchased from the retail market in Shiraz province and were examined by a specific PCR assay. This study showed positive 5/04% out of 238 unpasteurized dairy products including 9 out of 48 (18/75%) raw milk samples and 3 out of 48 (6.25%) yogurt samples). Contamination was not detected in samples of dough, cheese and traditional ice cream. The results also showed that among 12 positive samples, 6 samples were contaminated with B. abortus (including 4 milk samples and 2 yogurt samples), 2 samples were contaminated with B. melitensis (including 2 Milk samples) and 4 samples were contaminated simultaneously with B. abortus and B. melitensis (including 3 milk samples and 1 yogurt sample). The present study suggests the unpasteurized dairy products as the major sources of brucellosis in Shiraz province, South of Iran; thus, to prevent brucellosis in human, the consumption of pasteurized milk and dairy products is highly recommended.
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spelling pubmed-77277672020-12-11 Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay Abdali, Fargol Hosseinzadeh, Saeid Berizi, Enayat Pourmontaseri, Maryam Mol Biol Res Commun Original Article The consumption of milk and unpasteurized dairy products contaminated with Brucella bacteria is one of the most important ways of brucellosis transmission to humans. The principal goal of this study was to determine the prevalence of Brucella abortus (B. abortus) and Brucella melitens (B. melitens) in unpasteurized dairy products consumed in Shiraz province. In this study conducted in 2016, 238 unpasteurized dairy products including 48 raw milk, 48 yogurt, 46 cheeses, 48 dough and 48 ice cream samples, were purchased from the retail market in Shiraz province and were examined by a specific PCR assay. This study showed positive 5/04% out of 238 unpasteurized dairy products including 9 out of 48 (18/75%) raw milk samples and 3 out of 48 (6.25%) yogurt samples). Contamination was not detected in samples of dough, cheese and traditional ice cream. The results also showed that among 12 positive samples, 6 samples were contaminated with B. abortus (including 4 milk samples and 2 yogurt samples), 2 samples were contaminated with B. melitensis (including 2 Milk samples) and 4 samples were contaminated simultaneously with B. abortus and B. melitensis (including 3 milk samples and 1 yogurt sample). The present study suggests the unpasteurized dairy products as the major sources of brucellosis in Shiraz province, South of Iran; thus, to prevent brucellosis in human, the consumption of pasteurized milk and dairy products is highly recommended. Shiraz University 2020-09 /pmc/articles/PMC7727767/ /pubmed/33313331 http://dx.doi.org/10.22099/mbrc.2020.37381.1506 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Abdali, Fargol
Hosseinzadeh, Saeid
Berizi, Enayat
Pourmontaseri, Maryam
Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay
title Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay
title_full Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay
title_fullStr Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay
title_full_unstemmed Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay
title_short Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay
title_sort prevalence of brucella species in unpasteurized dairy products consumed in shiraz province using pcr assay
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727767/
https://www.ncbi.nlm.nih.gov/pubmed/33313331
http://dx.doi.org/10.22099/mbrc.2020.37381.1506
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