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Acetate and glycerol are not uniquely suited for the evolution of cross-feeding in E. coli

The evolution of cross-feeding among individuals of the same species can help generate genetic and phenotypic diversity even in completely homogeneous environments. Cross-feeding Escherichia coli strains, where one strain feeds on a carbon source excreted by another strain, rapidly emerge during exp...

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Detalles Bibliográficos
Autores principales: San Roman, Magdalena, Wagner, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728234/
https://www.ncbi.nlm.nih.gov/pubmed/33253183
http://dx.doi.org/10.1371/journal.pcbi.1008433
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author San Roman, Magdalena
Wagner, Andreas
author_facet San Roman, Magdalena
Wagner, Andreas
author_sort San Roman, Magdalena
collection PubMed
description The evolution of cross-feeding among individuals of the same species can help generate genetic and phenotypic diversity even in completely homogeneous environments. Cross-feeding Escherichia coli strains, where one strain feeds on a carbon source excreted by another strain, rapidly emerge during experimental evolution in a chemically minimal environment containing glucose as the sole carbon source. Genome-scale metabolic modeling predicts that cross-feeding of 58 carbon sources can emerge in the same environment, but only cross-feeding of acetate and glycerol has been experimentally observed. Here we use metabolic modeling to ask whether acetate and glycerol cross-feeding are especially likely to evolve, perhaps because they require less metabolic change, and thus perhaps also less genetic change than other cross-feeding interactions. However, this is not the case. The minimally required metabolic changes required for acetate and glycerol cross feeding affect dozens of chemical reactions, multiple biochemical pathways, as well as multiple operons or regulons. The complexity of these changes is consistent with experimental observations, where cross-feeding strains harbor multiple mutations. The required metabolic changes are also no less complex than those observed for multiple other of the 56 cross feeding interactions we study. We discuss possible reasons why only two cross-feeding interactions have been discovered during experimental evolution and argue that multiple new cross-feeding interactions may await discovery.
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spelling pubmed-77282342020-12-16 Acetate and glycerol are not uniquely suited for the evolution of cross-feeding in E. coli San Roman, Magdalena Wagner, Andreas PLoS Comput Biol Research Article The evolution of cross-feeding among individuals of the same species can help generate genetic and phenotypic diversity even in completely homogeneous environments. Cross-feeding Escherichia coli strains, where one strain feeds on a carbon source excreted by another strain, rapidly emerge during experimental evolution in a chemically minimal environment containing glucose as the sole carbon source. Genome-scale metabolic modeling predicts that cross-feeding of 58 carbon sources can emerge in the same environment, but only cross-feeding of acetate and glycerol has been experimentally observed. Here we use metabolic modeling to ask whether acetate and glycerol cross-feeding are especially likely to evolve, perhaps because they require less metabolic change, and thus perhaps also less genetic change than other cross-feeding interactions. However, this is not the case. The minimally required metabolic changes required for acetate and glycerol cross feeding affect dozens of chemical reactions, multiple biochemical pathways, as well as multiple operons or regulons. The complexity of these changes is consistent with experimental observations, where cross-feeding strains harbor multiple mutations. The required metabolic changes are also no less complex than those observed for multiple other of the 56 cross feeding interactions we study. We discuss possible reasons why only two cross-feeding interactions have been discovered during experimental evolution and argue that multiple new cross-feeding interactions may await discovery. Public Library of Science 2020-11-30 /pmc/articles/PMC7728234/ /pubmed/33253183 http://dx.doi.org/10.1371/journal.pcbi.1008433 Text en © 2020 San Roman, Wagner http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
San Roman, Magdalena
Wagner, Andreas
Acetate and glycerol are not uniquely suited for the evolution of cross-feeding in E. coli
title Acetate and glycerol are not uniquely suited for the evolution of cross-feeding in E. coli
title_full Acetate and glycerol are not uniquely suited for the evolution of cross-feeding in E. coli
title_fullStr Acetate and glycerol are not uniquely suited for the evolution of cross-feeding in E. coli
title_full_unstemmed Acetate and glycerol are not uniquely suited for the evolution of cross-feeding in E. coli
title_short Acetate and glycerol are not uniquely suited for the evolution of cross-feeding in E. coli
title_sort acetate and glycerol are not uniquely suited for the evolution of cross-feeding in e. coli
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728234/
https://www.ncbi.nlm.nih.gov/pubmed/33253183
http://dx.doi.org/10.1371/journal.pcbi.1008433
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