Cargando…

Generation and validation of versatile inducible CRISPRi embryonic stem cell and mouse model

Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated (Cas) 9 has been widely used far beyond genome editing. Fusions of deactivated Cas9 (dCas9) to transcription effectors enable interrogation of the epigenome and controlling of gene expression. However, the large tran...

Descripción completa

Detalles Bibliográficos
Autores principales:	Li, Rui, Xia, Xianyou, Wang, Xing, Sun, Xiaoyu, Dai, Zhongye, Huo, Dawei, Zheng, Huimin, Xiong, Haiqing, He, Aibin, Wu, Xudong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2020
Materias:	Methods and Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728392/ https://www.ncbi.nlm.nih.gov/pubmed/33253175 http://dx.doi.org/10.1371/journal.pbio.3000749

Ejemplares similares

A cloud-based toolbox for the versatile environmental annotation of biodiversity data
por: Li, Richard, et al.
Publicado: (2021)

PiVR: An affordable and versatile closed-loop platform to study unrestrained sensorimotor behavior
por: Tadres, David, et al.
Publicado: (2020)

PE-STOP: A versatile tool for installing nonsense substitutions amenable for precise reversion
por: Song, Ziguo, et al.
Publicado: (2023)

Single-cell RNA-seq analysis of mouse preimplantation embryos by third-generation sequencing
por: Fan, Xiaoying, et al.
Publicado: (2020)

Development of a versatile LCM-Seq method for spatial transcriptomics of fluorescently tagged cholinergic neuron populations
por: Rumpler, Éva, et al.
Publicado: (2023)

Targeted genetic screening in mice through haploid embryonic stem cells identifies critical genes in bone development
por: Bai, Meizhu, et al.
Publicado: (2019)

Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes
por: Allen, Sarah E., et al.
Publicado: (2021)

A versatile new tool derived from a bacterial deubiquitylase to detect and purify ubiquitylated substrates and their interacting proteins
por: Zhang, Mengwen, et al.
Publicado: (2022)

Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi
por: Jensen, Trine I., et al.
Publicado: (2021)

Construction and validation of customized genomes for human and mouse ribosomal DNA mapping
por: George, Subin S., et al.
Publicado: (2023)

A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum
por: Liang, Xiaoying, et al.
Publicado: (2022)

Improved discovery of genetic interactions using CRISPRiSeq across multiple environments
por: Jaffe, Mia, et al.
Publicado: (2019)

An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells
por: Harikumar, Arigela, et al.
Publicado: (2017)

A systems genetics resource and analysis of sleep regulation in the mouse
por: Diessler, Shanaz, et al.
Publicado: (2018)

CellProfiler 3.0: Next-generation image processing for biology
por: McQuin, Claire, et al.
Publicado: (2018)

Determination of globotriaosylceramide analogs in the organs of a mouse model of Fabry disease
por: Ishii, Satoshi, et al.
Publicado: (2020)

Thinking small: Next-generation sensor networks close the size gap in vertebrate biologging
por: Ripperger, Simon P., et al.
Publicado: (2020)

Meiotic Cas9 expression mediates gene conversion in the male and female mouse germline
por: Weitzel, Alexander J., et al.
Publicado: (2021)

HMEJ-based safe-harbor genome editing enables efficient generation of cattle with increased resistance to tuberculosis
por: Yuan, Mengke, et al.
Publicado: (2021)

Assessing the replicability of spatial gene expression using atlas data from the adult mouse brain
por: Lu, Shaina, et al.
Publicado: (2021)

Integrated transcriptomics and epigenomics reveal chamber-specific and species-specific characteristics of human and mouse hearts
por: Gao, Junpeng, et al.
Publicado: (2021)

scapGNN: A graph neural network–based framework for active pathway and gene module inference from single-cell multi-omics data
por: Han, Xudong, et al.
Publicado: (2023)

Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells
por: Shelton, Michael, et al.
Publicado: (2014)

Fast Quantitative Real-Time PCR-Based Screening for Common Chromosomal Aneuploidies in Mouse Embryonic Stem Cells
por: D’Hulst, Charlotte, et al.
Publicado: (2013)

Identifying Division Symmetry of Mouse Embryonic Stem Cells: Negative Impact of DNA Methyltransferases on Symmetric Self-Renewal
por: Jasnos, Lukasz, et al.
Publicado: (2013)

The sea cucumber genome provides insights into morphological evolution and visceral regeneration
por: Zhang, Xiaojun, et al.
Publicado: (2017)

CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces
por: Bai, Chaoxian, et al.
Publicado: (2023)

Rapid detection of SARS-CoV-2 with CRISPR-Cas12a
por: Xiong, Dan, et al.
Publicado: (2020)

Antibody validation for Western blot: By the user, for the user
por: Pillai-Kastoori, Lakshmi, et al.
Publicado: (2020)

ClipKIT: A multiple sequence alignment trimming software for accurate phylogenomic inference
por: Steenwyk, Jacob L., et al.
Publicado: (2020)

OrthoSNAP: A tree splitting and pruning algorithm for retrieving single-copy orthologs from gene family trees
por: Steenwyk, Jacob L., et al.
Publicado: (2022)

Precision Health Resource of Control iPSC Lines for Versatile Multilineage Differentiation
por: Hildebrandt, Matthew R., et al.
Publicado: (2019)

AliSim: A Fast and Versatile Phylogenetic Sequence Simulator for the Genomic Era
por: Ly-Trong, Nhan, et al.
Publicado: (2022)

DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes
por: Kuno, Akihiro, et al.
Publicado: (2022)

Development and validation of a high-throughput whole cell assay to investigate Staphylococcus aureus adhesion to host ligands
por: Petrie, Laurenne E., et al.
Publicado: (2021)

RefPlantNLR is a comprehensive collection of experimentally validated plant disease resistance proteins from the NLR family
por: Kourelis, Jiorgos, et al.
Publicado: (2021)

RNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1(+) Roof-Plate-Derived Cells
por: Ghazale, Hussein, et al.
Publicado: (2019)

Significant decrease of maternal mitochondria carryover using optimized spindle-chromosomal complex transfer
por: Liao, Xiaoyu, et al.
Publicado: (2023)

PhyloFisher: A phylogenomic package for resolving eukaryotic relationships
por: Tice, Alexander K., et al.
Publicado: (2021)

Cellular abundance of sodium phosphate cotransporter SLC20A1/PiT1 and phosphate uptake are controlled post-transcriptionally by ESCRT
por: Zechner, Christoph, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_1nae0d50lrq1tr5pbtmg09gssb