An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification

Green fluorescent proteins (GFPs) are widely used to monitor membrane protein expression, purification, and stability. An ideal reporter should be stable itself and provide high sensitivity and yield. Here, we demonstrate that a coral (Galaxea fascicularis) thermostable GFP (TGP) is by such reasons...

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Detalles Bibliográficos
Autores principales: Cai, Hongmin, Yao, Hebang, Li, Tingting, Hutter, Cedric A. J., Li, Yanfang, Tang, Yannan, Seeger, Markus A., Li, Dianfan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729955/
https://www.ncbi.nlm.nih.gov/pubmed/33303987
http://dx.doi.org/10.1038/s42003-020-01478-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729955/
https://www.ncbi.nlm.nih.gov/pubmed/33303987
http://dx.doi.org/10.1038/s42003-020-01478-z

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