The molecular basis for recognition of 5′-NNNCC-3′ PAM and its methylation state by Acidothermus cellulolyticus Cas9

Acidothermus cellulolyticus CRISPR-Cas9 (AceCas9) is a thermophilic Type II-C enzyme that has potential genome editing applications in extreme environments. It cleaves DNA with a 5′-NNNCC-3′ Protospacer Adjacent Motif (PAM) and is sensitive to its methylation status. To understand the molecular basi...

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Detalles Bibliográficos
Autores principales: Das, Anuska, Hand, Travis H., Smith, Chardasia L., Wickline, Ethan, Zawrotny, Michael, Li, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733487/
https://www.ncbi.nlm.nih.gov/pubmed/33311465
http://dx.doi.org/10.1038/s41467-020-20204-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733487/
https://www.ncbi.nlm.nih.gov/pubmed/33311465
http://dx.doi.org/10.1038/s41467-020-20204-1

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