Minimal system for assembly of SARS-CoV-2 virus like particles

SARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs possess S protein spikes on particle exterior, making...

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Autores principales: Swann, Heather, Sharma, Abhimanyu, Preece, Benjamin, Peterson, Abby, Eldredge, Crystal, Belnap, David M., Vershinin, Michael, Saffarian, Saveez
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736577/
https://www.ncbi.nlm.nih.gov/pubmed/33318562
http://dx.doi.org/10.1038/s41598-020-78656-w
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author Swann, Heather
Sharma, Abhimanyu
Preece, Benjamin
Peterson, Abby
Eldredge, Crystal
Belnap, David M.
Vershinin, Michael
Saffarian, Saveez
author_facet Swann, Heather
Sharma, Abhimanyu
Preece, Benjamin
Peterson, Abby
Eldredge, Crystal
Belnap, David M.
Vershinin, Michael
Saffarian, Saveez
author_sort Swann, Heather
collection PubMed
description SARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs possess S protein spikes on particle exterior, making them ideal for vaccine development. The particles range in shape from spherical to elongated with a characteristic size of 129 ± 32 nm. We further show that SARS-CoV-2 VLPs dried in ambient conditions can retain their structural integrity upon repeated scans with Atomic Force Microscopy up to a peak force of 1 nN.
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spelling pubmed-77365772020-12-15 Minimal system for assembly of SARS-CoV-2 virus like particles Swann, Heather Sharma, Abhimanyu Preece, Benjamin Peterson, Abby Eldredge, Crystal Belnap, David M. Vershinin, Michael Saffarian, Saveez Sci Rep Article SARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs possess S protein spikes on particle exterior, making them ideal for vaccine development. The particles range in shape from spherical to elongated with a characteristic size of 129 ± 32 nm. We further show that SARS-CoV-2 VLPs dried in ambient conditions can retain their structural integrity upon repeated scans with Atomic Force Microscopy up to a peak force of 1 nN. Nature Publishing Group UK 2020-12-14 /pmc/articles/PMC7736577/ /pubmed/33318562 http://dx.doi.org/10.1038/s41598-020-78656-w Text en © The Author(s) 2020, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Swann, Heather
Sharma, Abhimanyu
Preece, Benjamin
Peterson, Abby
Eldredge, Crystal
Belnap, David M.
Vershinin, Michael
Saffarian, Saveez
Minimal system for assembly of SARS-CoV-2 virus like particles
title Minimal system for assembly of SARS-CoV-2 virus like particles
title_full Minimal system for assembly of SARS-CoV-2 virus like particles
title_fullStr Minimal system for assembly of SARS-CoV-2 virus like particles
title_full_unstemmed Minimal system for assembly of SARS-CoV-2 virus like particles
title_short Minimal system for assembly of SARS-CoV-2 virus like particles
title_sort minimal system for assembly of sars-cov-2 virus like particles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736577/
https://www.ncbi.nlm.nih.gov/pubmed/33318562
http://dx.doi.org/10.1038/s41598-020-78656-w
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