Cargando…
Cepharanthine as a Potential Novel Tumor-Regional Therapy in Treating Cutaneous Melanoma: Altering the Expression of Cathepsin B, Tumor Suppressor Genes and Autophagy-Related Proteins
The incidence of primary cutaneous melanoma continues to increase annually and is one of the most aggressive malignancies in humans and need to develop more novel non-surgical therapies. Autophagy and cathepsin B targeted therapy was reported to improve melanoma treatment. Cepharanthine (CEP), a nat...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736638/ https://www.ncbi.nlm.nih.gov/pubmed/33335896 http://dx.doi.org/10.3389/fbioe.2020.601969 |
_version_ | 1783622805737177088 |
---|---|
author | Liu, Yufang Xie, Yang Lin, Yao Xu, Qingfang Huang, Yunfen Peng, Mengran Lai, Wei Zheng, Yue |
author_facet | Liu, Yufang Xie, Yang Lin, Yao Xu, Qingfang Huang, Yunfen Peng, Mengran Lai, Wei Zheng, Yue |
author_sort | Liu, Yufang |
collection | PubMed |
description | The incidence of primary cutaneous melanoma continues to increase annually and is one of the most aggressive malignancies in humans and need to develop more novel non-surgical therapies. Autophagy and cathepsin B targeted therapy was reported to improve melanoma treatment. Cepharanthine (CEP), a natural alkaloid extracted from the genus Cephalophyllum has been reported to have the function of inhibiting cancers. We found that CEP inhibited human primary cutaneous melanoma cells viability and proliferation in 24 h in vitro, and topical application or intra-tumoral injection of CEP decreased the growth of cutaneous melanoma in mice within 4 weeks. CEP preparations below 50% concentration did not induce skin irritation and allergy reaction on human skin in vivo. Primary cutaneous melanoma cells incubated with CEP, the expression of cathepsin B was decreased and the LC3-I and LC3-II expression changed in a dose-dependent manner, while p53, p21Cip1p, and p16Inka gene expression was up-regulated. We demonstrated the effects of CEP as a novel tumor-regional therapy for cutaneous melanoma and provided a preliminary research basis for future clinical treatment researches and the exploration of integrated treatments with systemic therapy, radiotherapy, and surgery for human primary cutaneous melanoma. |
format | Online Article Text |
id | pubmed-7736638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77366382020-12-16 Cepharanthine as a Potential Novel Tumor-Regional Therapy in Treating Cutaneous Melanoma: Altering the Expression of Cathepsin B, Tumor Suppressor Genes and Autophagy-Related Proteins Liu, Yufang Xie, Yang Lin, Yao Xu, Qingfang Huang, Yunfen Peng, Mengran Lai, Wei Zheng, Yue Front Bioeng Biotechnol Bioengineering and Biotechnology The incidence of primary cutaneous melanoma continues to increase annually and is one of the most aggressive malignancies in humans and need to develop more novel non-surgical therapies. Autophagy and cathepsin B targeted therapy was reported to improve melanoma treatment. Cepharanthine (CEP), a natural alkaloid extracted from the genus Cephalophyllum has been reported to have the function of inhibiting cancers. We found that CEP inhibited human primary cutaneous melanoma cells viability and proliferation in 24 h in vitro, and topical application or intra-tumoral injection of CEP decreased the growth of cutaneous melanoma in mice within 4 weeks. CEP preparations below 50% concentration did not induce skin irritation and allergy reaction on human skin in vivo. Primary cutaneous melanoma cells incubated with CEP, the expression of cathepsin B was decreased and the LC3-I and LC3-II expression changed in a dose-dependent manner, while p53, p21Cip1p, and p16Inka gene expression was up-regulated. We demonstrated the effects of CEP as a novel tumor-regional therapy for cutaneous melanoma and provided a preliminary research basis for future clinical treatment researches and the exploration of integrated treatments with systemic therapy, radiotherapy, and surgery for human primary cutaneous melanoma. Frontiers Media S.A. 2020-12-01 /pmc/articles/PMC7736638/ /pubmed/33335896 http://dx.doi.org/10.3389/fbioe.2020.601969 Text en Copyright © 2020 Liu, Xie, Lin, Xu, Huang, Peng, Lai and Zheng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Liu, Yufang Xie, Yang Lin, Yao Xu, Qingfang Huang, Yunfen Peng, Mengran Lai, Wei Zheng, Yue Cepharanthine as a Potential Novel Tumor-Regional Therapy in Treating Cutaneous Melanoma: Altering the Expression of Cathepsin B, Tumor Suppressor Genes and Autophagy-Related Proteins |
title | Cepharanthine as a Potential Novel Tumor-Regional Therapy in Treating Cutaneous Melanoma: Altering the Expression of Cathepsin B, Tumor Suppressor Genes and Autophagy-Related Proteins |
title_full | Cepharanthine as a Potential Novel Tumor-Regional Therapy in Treating Cutaneous Melanoma: Altering the Expression of Cathepsin B, Tumor Suppressor Genes and Autophagy-Related Proteins |
title_fullStr | Cepharanthine as a Potential Novel Tumor-Regional Therapy in Treating Cutaneous Melanoma: Altering the Expression of Cathepsin B, Tumor Suppressor Genes and Autophagy-Related Proteins |
title_full_unstemmed | Cepharanthine as a Potential Novel Tumor-Regional Therapy in Treating Cutaneous Melanoma: Altering the Expression of Cathepsin B, Tumor Suppressor Genes and Autophagy-Related Proteins |
title_short | Cepharanthine as a Potential Novel Tumor-Regional Therapy in Treating Cutaneous Melanoma: Altering the Expression of Cathepsin B, Tumor Suppressor Genes and Autophagy-Related Proteins |
title_sort | cepharanthine as a potential novel tumor-regional therapy in treating cutaneous melanoma: altering the expression of cathepsin b, tumor suppressor genes and autophagy-related proteins |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736638/ https://www.ncbi.nlm.nih.gov/pubmed/33335896 http://dx.doi.org/10.3389/fbioe.2020.601969 |
work_keys_str_mv | AT liuyufang cepharanthineasapotentialnoveltumorregionaltherapyintreatingcutaneousmelanomaalteringtheexpressionofcathepsinbtumorsuppressorgenesandautophagyrelatedproteins AT xieyang cepharanthineasapotentialnoveltumorregionaltherapyintreatingcutaneousmelanomaalteringtheexpressionofcathepsinbtumorsuppressorgenesandautophagyrelatedproteins AT linyao cepharanthineasapotentialnoveltumorregionaltherapyintreatingcutaneousmelanomaalteringtheexpressionofcathepsinbtumorsuppressorgenesandautophagyrelatedproteins AT xuqingfang cepharanthineasapotentialnoveltumorregionaltherapyintreatingcutaneousmelanomaalteringtheexpressionofcathepsinbtumorsuppressorgenesandautophagyrelatedproteins AT huangyunfen cepharanthineasapotentialnoveltumorregionaltherapyintreatingcutaneousmelanomaalteringtheexpressionofcathepsinbtumorsuppressorgenesandautophagyrelatedproteins AT pengmengran cepharanthineasapotentialnoveltumorregionaltherapyintreatingcutaneousmelanomaalteringtheexpressionofcathepsinbtumorsuppressorgenesandautophagyrelatedproteins AT laiwei cepharanthineasapotentialnoveltumorregionaltherapyintreatingcutaneousmelanomaalteringtheexpressionofcathepsinbtumorsuppressorgenesandautophagyrelatedproteins AT zhengyue cepharanthineasapotentialnoveltumorregionaltherapyintreatingcutaneousmelanomaalteringtheexpressionofcathepsinbtumorsuppressorgenesandautophagyrelatedproteins |