Directed co-evolution of interacting protein–peptide pairs by compartmentalized two-hybrid replication (C2HR)

Directed evolution methodologies benefit from read-outs quantitatively linking genotype to phenotype. We therefore devised a method that couples protein–peptide interactions to the dynamic read-out provided by an engineered DNA polymerase. Fusion of a processivity clamp protein to a thermostable nuc...

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Detalles Bibliográficos
Autores principales: Siau, Jia Wei, Nonis, Samuel, Chee, Sharon, Koh, Li Quan, Ferrer, Fernando J, Brown, Christopher J, Ghadessy, Farid J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736784/
https://www.ncbi.nlm.nih.gov/pubmed/33104786
http://dx.doi.org/10.1093/nar/gkaa933

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736784/
https://www.ncbi.nlm.nih.gov/pubmed/33104786
http://dx.doi.org/10.1093/nar/gkaa933

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