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Directed co-evolution of interacting protein–peptide pairs by compartmentalized two-hybrid replication (C2HR)
Directed evolution methodologies benefit from read-outs quantitatively linking genotype to phenotype. We therefore devised a method that couples protein–peptide interactions to the dynamic read-out provided by an engineered DNA polymerase. Fusion of a processivity clamp protein to a thermostable nuc...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736784/ https://www.ncbi.nlm.nih.gov/pubmed/33104786 http://dx.doi.org/10.1093/nar/gkaa933 |