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A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”

A chemiluminescent immunoassay for human serum osteocalcin, or bone Gla protein, was established using a double-antibody sandwich model. Examination of the hook effect revealed that it was significantly reduced, and no hook effect was observed at an osteocalcin concentration of 4000 ng/mL. The estab...

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Autores principales: Han, Shuang, Xue, Yifeng, Zhang, Junlan, Huang, Jianrong, Liu, Xiuxia, Yang, Yankun, Bai, Zhonghu, Wang, Chunxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744234/
https://www.ncbi.nlm.nih.gov/pubmed/33376621
http://dx.doi.org/10.1155/2020/8891437
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author Han, Shuang
Xue, Yifeng
Zhang, Junlan
Huang, Jianrong
Liu, Xiuxia
Yang, Yankun
Bai, Zhonghu
Wang, Chunxin
author_facet Han, Shuang
Xue, Yifeng
Zhang, Junlan
Huang, Jianrong
Liu, Xiuxia
Yang, Yankun
Bai, Zhonghu
Wang, Chunxin
author_sort Han, Shuang
collection PubMed
description A chemiluminescent immunoassay for human serum osteocalcin, or bone Gla protein, was established using a double-antibody sandwich model. Examination of the hook effect revealed that it was significantly reduced, and no hook effect was observed at an osteocalcin concentration of 4000 ng/mL. The established method showed good analytical performance and thermal stability. The limit of detection was 0.03 ng/mL. The intra-assay coefficient of variation (CV) was 3.22%–5.64%, the interassay CV was 4.42%–7.25%, and the recovery rate was 93.22%–107.99%. Cross-reactivity (CR) was not observed with bovine, rat, mouse, rabbit, or porcine samples. The developed method showed a good correlation with the N-MID product from Roche. In total, 1069 clinical patient samples were measured using the reagent kit developed in this study.
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spelling pubmed-77442342020-12-28 A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect” Han, Shuang Xue, Yifeng Zhang, Junlan Huang, Jianrong Liu, Xiuxia Yang, Yankun Bai, Zhonghu Wang, Chunxin J Anal Methods Chem Research Article A chemiluminescent immunoassay for human serum osteocalcin, or bone Gla protein, was established using a double-antibody sandwich model. Examination of the hook effect revealed that it was significantly reduced, and no hook effect was observed at an osteocalcin concentration of 4000 ng/mL. The established method showed good analytical performance and thermal stability. The limit of detection was 0.03 ng/mL. The intra-assay coefficient of variation (CV) was 3.22%–5.64%, the interassay CV was 4.42%–7.25%, and the recovery rate was 93.22%–107.99%. Cross-reactivity (CR) was not observed with bovine, rat, mouse, rabbit, or porcine samples. The developed method showed a good correlation with the N-MID product from Roche. In total, 1069 clinical patient samples were measured using the reagent kit developed in this study. Hindawi 2020-12-09 /pmc/articles/PMC7744234/ /pubmed/33376621 http://dx.doi.org/10.1155/2020/8891437 Text en Copyright © 2020 Shuang Han et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Han, Shuang
Xue, Yifeng
Zhang, Junlan
Huang, Jianrong
Liu, Xiuxia
Yang, Yankun
Bai, Zhonghu
Wang, Chunxin
A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”
title A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”
title_full A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”
title_fullStr A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”
title_full_unstemmed A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”
title_short A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”
title_sort chemiluminescent immunoassay for osteocalcin in human serum and a solution to the “hook effect”
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744234/
https://www.ncbi.nlm.nih.gov/pubmed/33376621
http://dx.doi.org/10.1155/2020/8891437
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