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Pre-amplification as a method for improvement of quantitative RT-PCR analysis of circulating miRNAs

INTRODUCTION: The assessment of circulating miRNAs is challenging and still limited due to their low concentrations, small size and lack of reference values in human biological samples. Pre-amplification of complementary DNAs may facilitate reliable miRNA quantification. The aim of our study was to...

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Detalles Bibliográficos
Autores principales: Sekovanić, Ankica, Dorotić, Adrijana, Jurasović, Jasna, Pašalić, Daria, Kovačić, Jelena, Stasenko, Sandra, Mioč, Tatjana, Piasek, Martina, Orct, Tatjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Croatian Society of Medical Biochemistry and Laboratory Medicine 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745165/
https://www.ncbi.nlm.nih.gov/pubmed/33380895
http://dx.doi.org/10.11613/BM.2021.010901