Cargando…

Pre-amplification as a method for improvement of quantitative RT-PCR analysis of circulating miRNAs

INTRODUCTION: The assessment of circulating miRNAs is challenging and still limited due to their low concentrations, small size and lack of reference values in human biological samples. Pre-amplification of complementary DNAs may facilitate reliable miRNA quantification. The aim of our study was to...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sekovanić, Ankica, Dorotić, Adrijana, Jurasović, Jasna, Pašalić, Daria, Kovačić, Jelena, Stasenko, Sandra, Mioč, Tatjana, Piasek, Martina, Orct, Tatjana
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Croatian Society of Medical Biochemistry and Laboratory Medicine 2020
Materias:	Short Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745165/ https://www.ncbi.nlm.nih.gov/pubmed/33380895 http://dx.doi.org/10.11613/BM.2021.010901

Ejemplares similares

The effects of maternal cigarette smoking on cadmium and lead levels, miRNA expression and biochemical parameters across the feto-placental unit
por: Sekovanić, Ankica, et al.
Publicado: (2022)

Mercury Exposure Assessment in Mother–Infant Pairs from Continental and Coastal Croatia
por: Sekovanić, Ankica, et al.
Publicado: (2020)

Cigarette Smoking during Pregnancy: Effects on Antioxidant Enzymes, Metallothionein and Trace Elements in Mother-Newborn Pairs
por: Pizent, Alica, et al.
Publicado: (2020)

Metallothionein 2A Gene Polymorphisms in Relation to Diseases and Trace Element Levels in Humans
por: Sekovanić, Ankica, et al.
Publicado: (2020)

Mercury Disposition in Suckling Rats: Comparative Assessment Following Parenteral Exposure to Thiomersal and Mercuric Chloride
por: Blanuša, Maja, et al.
Publicado: (2012)

Effects of Maternal Cigarette Smoking on Trace Element Levels and Steroidogenesis in the Maternal–Placental–Fetal Unit
por: Piasek, Martina, et al.
Publicado: (2023)

The Effects of Chromium and Vanadium on Biomarkers of Carbohydrate and Lipid Metabolism in Workers Exposed to Coal Fly Ash
por: Zeneli, Lulzim, et al.
Publicado: (2022)

Your article needs revision? – How to improve it successfully and answer to the reviewers’ comments
por: Pašalić, Daria, et al.
Publicado: (2021)

Effects of Sub-Chronic Exposure to Imidacloprid on Reproductive Organs of Adult Male Rats: Antioxidant State, DNA Damage, and Levels of Essential Elements
por: Tariba Lovaković, Blanka, et al.
Publicado: (2021)

Potentially Toxic Elements in Water, Sediments and Fish from the Karstic River (Raša River, Croatia) Located in the Former Coal-Mining Area
por: Kljaković-Gašpić, Zorana, et al.
Publicado: (2022)

Genetic and Epigenetic Features of Uveal Melanoma—An Overview and Clinical Implications
por: Pašalić, Daria, et al.
Publicado: (2023)

Diurnal Salivary Cortisol in Relation to Body Composition and Heart Rate Variability in Young Adults
por: Cvijetic, Selma, et al.
Publicado: (2022)

Heavy Metals and Essential Metals Are Associated with Cerebrospinal Fluid Biomarkers of Alzheimer’s Disease
por: Babić Leko, Mirjana, et al.
Publicado: (2022)

External quality assessment of medical laboratories in Croatia: preliminary evaluation of post-analytical laboratory testing
por: Krleza, Jasna Lenicek, et al.
Publicado: (2017)

Capillary blood sampling: national recommendations on behalf of the Croatian Society of Medical Biochemistry and Laboratory Medicine
por: Krleza, Jasna Lenicek, et al.
Publicado: (2015)

The Impact of Long-Term Clinoptilolite Administration on the Concentration Profile of Metals in Rodent Organisms
por: Dolanc, Ivan, et al.
Publicado: (2023)

Comparison of the RNA-amplification based methods RT–PCR and NASBA for the detection of circulating tumour cells
por: Burchill, S A, et al.
Publicado: (2002)

Evaluation of extraction kits and RT-qPCR systems adapted to high-throughput platform for circulating miRNAs
por: Tan, Geok Wee, et al.
Publicado: (2015)

Clinical COVID-19 diagnostic methods: Comparison of reverse transcription loop-mediated isothermal amplification (RT-LAMP) and quantitative RT-PCR (qRT-PCR)
por: Kitajima, Heita, et al.
Publicado: (2021)

In female rats, ethylene glycol treatment elevates protein expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) without inducing hyperoxaluria
por: Breljak, Davorka, et al.
Publicado: (2015)

Blood gas testing and related measurements: National recommendations on behalf of the Croatian Society of Medical Biochemistry and Laboratory Medicine
por: Dukić, Lora, et al.
Publicado: (2016)

Identification of canine circulating miRNAs as tumor biospecific markers using Next-Generation Sequencing and Q-RT-PCR
por: Agarwal, Payal, et al.
Publicado: (2021)

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
por: Balcells, Ingrid, et al.
Publicado: (2011)

Multicenter Evaluation of Independent High-Throughput and RT-qPCR Technologies for the Development of Analytical Workflows for Circulating miRNA Analysis
por: Babayan, Anna, et al.
Publicado: (2020)

Optimized RT-qPCR and a novel normalization method for validating circulating miRNA biomarkers in ageing-related diseases
por: Want, Andrew, et al.
Publicado: (2023)

Web-based LinRegPCR: application for the visualization and analysis of (RT)-qPCR amplification and melting data
por: Untergasser, Andreas, et al.
Publicado: (2021)

Hemolysis from a nurses’ standpoint – survey from four Croatian hospitals
por: Dorotić, Adrijana, et al.
Publicado: (2015)

A systemic approach to screening high-throughput RT-qPCR data for a suitable set of reference circulating miRNAs
por: Pagacz, Konrad, et al.
Publicado: (2020)

Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency
por: Ban, Eunmi, et al.
Publicado: (2021)

Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR
por: Berthet, Nicolas, et al.
Publicado: (2008)

Validation of extracellular miRNA quantification in blood samples using RT‐qPCR
por: Fauth, Maria, et al.
Publicado: (2019)

Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR
por: Ban, Eunmi, et al.
Publicado: (2022)

Human Circulating miRNAs Real-time qRT-PCR-based Analysis: An Overview of Endogenous Reference Genes Used for Data Normalization
por: Donati, Simone, et al.
Publicado: (2019)

Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3′-MicroRNA Isoforms
por: Nejad, Charlotte, et al.
Publicado: (2018)

Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis
por: Jaeger, Lauren Hubert, et al.
Publicado: (2021)

Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis
por: Chen, Yongxin, et al.
Publicado: (2009)

Selection of reference genes for miRNA qRT-PCR under abiotic stress in grapevine
por: Luo, Meng, et al.
Publicado: (2018)

Identification of Endogenous Control miRNAs for RT-qPCR in T-Cell Acute Lymphoblastic Leukemia
por: Drobna, Monika, et al.
Publicado: (2018)

Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples
por: Yu, Xuelian, et al.
Publicado: (2014)

Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR
por: Strati, Areti, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_g3ns9acoi00okj4ks822tj3kso