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A simple method using CRISPR-Cas9 to knock-out genes in murine cancerous cell lines
CRISPR-Cas9 system can be used to generate knock-out cancer cell lines. An insertion or deletion induced by a single guide RNA (gRNA) is often used to generate knock-out cells, however, some cells express the target gene by skipping the disrupted exon, or by using a splicing variant, thus losing the...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749119/ https://www.ncbi.nlm.nih.gov/pubmed/33339985 http://dx.doi.org/10.1038/s41598-020-79303-0 |