Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos

The CRISPR/Cas9 genome editing tool has the potential to improve the livestock breeding industry by allowing for the introduction of desirable traits. Although an efficient and targeted tool, the CRISPR/Cas9 system can have some drawbacks, including off-target mutations and mosaicism, particularly w...

Descripción completa

Detalles Bibliográficos
Autores principales: Hennig, Sadie L., Owen, Joseph R., Lin, Jason C., Young, Amy E., Ross, Pablo J., Van Eenennaam, Alison L., Murray, James D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749171/
https://www.ncbi.nlm.nih.gov/pubmed/33339870
http://dx.doi.org/10.1038/s41598-020-78264-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749171/
https://www.ncbi.nlm.nih.gov/pubmed/33339870
http://dx.doi.org/10.1038/s41598-020-78264-8

Ejemplares similares