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Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos

The CRISPR/Cas9 genome editing tool has the potential to improve the livestock breeding industry by allowing for the introduction of desirable traits. Although an efficient and targeted tool, the CRISPR/Cas9 system can have some drawbacks, including off-target mutations and mosaicism, particularly w...

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Detalles Bibliográficos
Autores principales:	Hennig, Sadie L., Owen, Joseph R., Lin, Jason C., Young, Amy E., Ross, Pablo J., Van Eenennaam, Alison L., Murray, James D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749171/ https://www.ncbi.nlm.nih.gov/pubmed/33339870 http://dx.doi.org/10.1038/s41598-020-78264-8

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