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Driving integrative structural modeling with serial capture affinity purification

Streamlined characterization of protein complexes remains a challenge for the study of protein interaction networks. Here we describe serial capture affinity purification (SCAP), in which two separate proteins are tagged with either the HaloTag or the SNAP-tag, permitting a multistep affinity enrich...

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Detalles Bibliográficos
Autores principales: Liu, Xingyu, Zhang, Ying, Wen, Zhihui, Hao, Yan, Banks, Charles A. S., Lange, Jeffrey J., Slaughter, Brian D., Unruh, Jay R., Florens, Laurence, Abmayr, Susan M., Workman, Jerry L., Washburn, Michael P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749342/
https://www.ncbi.nlm.nih.gov/pubmed/33257578
http://dx.doi.org/10.1073/pnas.2007931117