Cargando…

Driving integrative structural modeling with serial capture affinity purification

Streamlined characterization of protein complexes remains a challenge for the study of protein interaction networks. Here we describe serial capture affinity purification (SCAP), in which two separate proteins are tagged with either the HaloTag or the SNAP-tag, permitting a multistep affinity enrich...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Xingyu, Zhang, Ying, Wen, Zhihui, Hao, Yan, Banks, Charles A. S., Lange, Jeffrey J., Slaughter, Brian D., Unruh, Jay R., Florens, Laurence, Abmayr, Susan M., Workman, Jerry L., Washburn, Michael P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	National Academy of Sciences 2020
Materias:	Biological Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749342/ https://www.ncbi.nlm.nih.gov/pubmed/33257578 http://dx.doi.org/10.1073/pnas.2007931117

Ejemplares similares

WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
por: Gilmore, Joshua M., et al.
Publicado: (2016)

Characterization of a metazoan ADA acetyltransferase complex
por: Soffers, Jelly H M, et al.
Publicado: (2019)

Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration
por: Mohan, Ryan D., et al.
Publicado: (2014)

The disordered regions of the methyltransferase SETD2 govern its function by regulating its proteolysis and phase separation
por: Bhattacharya, Saikat, et al.
Publicado: (2021)

The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition
por: Huang, Fu, et al.
Publicado: (2016)

Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes
por: Lee, Kenneth K, et al.
Publicado: (2009)

Enzymatic modules of the SAGA chromatin-modifying complex play distinct roles in Drosophila gene expression and development
por: Li, Xuanying, et al.
Publicado: (2017)

Yeast Nuak1 phosphorylates histone H3 threonine 11 in low glucose stress by the cooperation of AMPK and CK2 signaling
por: Oh, Seunghee, et al.
Publicado: (2020)

Drosophila models reveal novel insights into mechanisms underlying neurodegeneration
por: Mohan, Ryan D, et al.
Publicado: (2014)

Pulling complexes out of complex diseases: Spinocerebellar Ataxia 7
por: Mohan, Ryan D, et al.
Publicado: (2014)

The plasticity of the pyruvate dehydrogenase complex confers a labile structure that is associated with its catalytic activity
por: Lee, Jaehyoun, et al.
Publicado: (2020)

The methyltransferase SETD2 couples transcription and splicing by engaging mRNA processing factors through its SHI domain
por: Bhattacharya, Saikat, et al.
Publicado: (2021)

HP1a Targets the Drosophila KDM4A Demethylase to a Subset of Heterochromatic Genes to Regulate H3K36me3 Levels
por: Lin, Chia-Hui, et al.
Publicado: (2012)

The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex
por: Hollopeter, Gunther, et al.
Publicado: (2014)

Reading and Interpreting the Histone Acylation Code
por: Soffers, Jelly H.M., et al.
Publicado: (2016)

Determining Protein Complex Connectivity Using a Probabilistic Deletion Network Derived from Quantitative Proteomics
por: Sardiu, Mihaela E., et al.
Publicado: (2009)

Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange
por: Smolle, Michaela, et al.
Publicado: (2012)

Proteins interacting with cloning scars: a source of false positive protein-protein interactions
por: Banks, Charles A. S., et al.
Publicado: (2015)

Swi/Snf dynamics on stress-responsive genes is governed by competitive bromodomain interactions
por: Dutta, Arnob, et al.
Publicado: (2014)

Non-Affinity Purification of Antibodies
por: Arakawa, Tsutomu, et al.
Publicado: (2023)

Sequential actin-based pushing forces drive meiosis I chromosome migration and symmetry breaking in oocytes
por: Yi, Kexi, et al.
Publicado: (2013)

BAC Modification through Serial or Simultaneous Use of CRE/Lox Technology
por: Parrish, Mark, et al.
Publicado: (2011)

Integrative Modeling of a Sin3/HDAC Complex Sub-structure
por: Banks, Charles A.S., et al.
Publicado: (2020)

Histone acetyltransferase Enok regulates oocyte polarization by promoting expression of the actin nucleation factor spire
por: Huang, Fu, et al.
Publicado: (2014)

Heparin affinity purification of extracellular vesicles
por: Balaj, Leonora, et al.
Publicado: (2015)

Affinity Membranes and Monoliths for Protein Purification
por: Lalli, Eleonora, et al.
Publicado: (2019)

Combinatorial depletion analysis to assemble the network architecture of the SAGA and ADA chromatin remodeling complexes
por: Lee, Kenneth K, et al.
Publicado: (2011)

Chromatin remodeller Fun30(Fft3) induces nucleosome disassembly to facilitate RNA polymerase II elongation
por: Lee, Junwoo, et al.
Publicado: (2017)

The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors
por: Dhatchinamoorthy, Karthik, et al.
Publicado: (2019)

Structural basis of the interaction between SETD2 methyltransferase and hnRNP L paralogs for governing co-transcriptional splicing
por: Bhattacharya, Saikat, et al.
Publicado: (2021)

Production and Purification of Streptokinase by Protected Affinity Chromatography
por: Babashamsi, Mohammad, et al.
Publicado: (2009)

Identification of sitagliptin binding proteins by affinity purification mass spectrometry: Sitagliptin binding proteins identified by affinity purification mass spectrometry
por: Wang, Xue-Ning, et al.
Publicado: (2022)

The CRAPome: a Contaminant Repository for Affinity Purification Mass Spectrometry Data
por: Mellacheruvu, Dattatreya, et al.
Publicado: (2013)

Serial interactome capture of the human cell nucleus
por: Conrad, Thomas, et al.
Publicado: (2016)

Structural plasticity of the living kinetochore
por: Dhatchinamoorthy, Karthik, et al.
Publicado: (2017)

Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification
por: Fraseur, Julia G., et al.
Publicado: (2018)

Affinity and Pseudo-Affinity Membrane Chromatography for Viral Vector and Vaccine Purifications: A Review
por: Lothert, Keven, et al.
Publicado: (2023)

HOXA1 and TALE proteins display cross-regulatory interactions and form a combinatorial binding code on HOXA1 targets
por: De Kumar, Bony, et al.
Publicado: (2017)

Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly
por: Banks, Charles A. S., et al.
Publicado: (2018)

Affinity Purification of an Archaeal DNA Replication Protein Network
por: Li, Zhuo, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_sogvc6uaoei6jhp2bdi8auug68