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Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins

Advanced fluorescence microscopy studies require specific and monovalent molecular labeling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labeled nanobodies against commonly employed fluorescent proteins (FPs). However, very little is known how t...

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Detalles Bibliográficos
Autores principales: Schneider, Falk, Sych, Taras, Eggeling, Christian, Sezgin, Erdinc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753935/
https://www.ncbi.nlm.nih.gov/pubmed/33364580
http://dx.doi.org/10.1016/j.isci.2020.101891