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Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins
Advanced fluorescence microscopy studies require specific and monovalent molecular labeling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labeled nanobodies against commonly employed fluorescent proteins (FPs). However, very little is known how t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753935/ https://www.ncbi.nlm.nih.gov/pubmed/33364580 http://dx.doi.org/10.1016/j.isci.2020.101891 |