In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9

In vivo mutagenesis systems accelerate directed protein evolution but often show restricted capabilities and deleterious off-site mutations on cells. To overcome these limitations, here we report an in vivo platform to diversify specific DNA segments based on protein fusions between various base dea...

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Detalles Bibliográficos
Autores principales: Álvarez, Beatriz, Mencía, Mario, de Lorenzo, Víctor, Fernández, Luis Ángel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755918/
https://www.ncbi.nlm.nih.gov/pubmed/33353963
http://dx.doi.org/10.1038/s41467-020-20230-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755918/
https://www.ncbi.nlm.nih.gov/pubmed/33353963
http://dx.doi.org/10.1038/s41467-020-20230-z

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